Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Gijs J L, Wuite"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-11 (2024)
Abstract Despite extensive studies on DNA replication, the exchange mechanisms of DNA polymerase during replication remain unclear. Existing models propose that this exchange is facilitated by protein partners like helicase. Here we present data, emp
Externí odkaz:
https://doaj.org/article/3d5c500a721b415dbb08911b751ff116
Autor:
Giulia Bergamaschi, Kees-Karel H. Taris, Andreas S. Biebricher, Xamanie M. R. Seymonson, Hannes Witt, Erwin J. G. Peterman, Gijs J. L. Wuite
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-14 (2024)
Abstract In the context of soft matter and cellular mechanics, microrheology - the use of micron-sized particles to probe the frequency-dependent viscoelastic response of materials – is widely used to shed light onto the mechanics and dynamics of m
Externí odkaz:
https://doaj.org/article/c22a827d38e146d699f5b30474cba1d6
Autor:
Dian Spakman, Tinka V. M. Clement, Andreas S. Biebricher, Graeme A. King, Manika I. Singh, Ian D. Hickson, Erwin J. G. Peterman, Gijs J. L. Wuite
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
In anaphase, any unresolved DNA entanglements between the segregating sister chromatids can give rise to chromatin bridges. Here, the authors present an in vitro single-molecule assay that mimics chromatin under tension, to show that PICH is a tensio
Externí odkaz:
https://doaj.org/article/a446e33a0b39439f9380f36b5bf5fe55
Autor:
Julia A. M. Bakx, Andreas S. Biebricher, Graeme A. King, Panagiotis Christodoulis, Kata Sarlós, Anna H. Bizard, Ian D. Hickson, Gijs J. L. Wuite, Erwin J. G. Peterman
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
Here the authors probe the cleavage and gate opening of single-stranded DNA by the human topoisomerase TRR using a unique single-molecule strategy to reveal structural plasticity in response to both double-stranded DNA and the helicase BLM.
Externí odkaz:
https://doaj.org/article/764b1c9c1010460d8e514df34cefdef4
Publikováno v:
Frontiers in Chemistry, Vol 10 (2022)
The economical and societal impact of COVID-19 has made the development of vaccines and drugs to combat SARS-CoV-2 infection a priority. While the SARS-CoV-2 spike protein has been widely explored as a drug target, the SARS-CoV-2 helicase (nsp13) doe
Externí odkaz:
https://doaj.org/article/16550a74f2184770aad41d3ed1a5db68
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
Abstract Architectural DNA–binding proteins are involved in many important DNA transactions by virtue of their ability to change DNA conformation. Histone-like protein from E. coli strain U93, HU, is one of the most studied bacterial architectural
Externí odkaz:
https://doaj.org/article/fd07cee0f0fd48a4b18836862b76e6ba
Autor:
Elya Dekel, Dana Yaffe, Irit Rosenhek-Goldian, Gili Ben-Nissan, Yifat Ofir-Birin, Mattia I. Morandi, Tamar Ziv, Xavier Sisquella, Matthew A. Pimentel, Thomas Nebl, Eugene Kapp, Yael Ohana Daniel, Paula Abou Karam, Daniel Alfandari, Ron Rotkopf, Shimrit Malihi, Tal Block Temin, Debakshi Mullick, Or-Yam Revach, Ariel Rudik, Nir S. Gov, Ido Azuri, Ziv Porat, Giulia Bergamaschi, Raya Sorkin, Gijs J. L. Wuite, Ori Avinoam, Teresa G. Carvalho, Sidney R. Cohen, Michal Sharon, Neta Regev-Rudzki
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
Plasmodium falciparum secretes extracellular vesicles (EVs) while growing inside red blood cells (RBCs). Here the authors show that these EVs contain assembled and functional 20S proteasome complexes that remodel the cytoskeleton of naïve human RBCs
Externí odkaz:
https://doaj.org/article/2714bdc7c6d440079ba403f2120173c1
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 3, p 2806 (2023)
Single-stranded DNA-binding proteins (SSBs) play vital roles in DNA metabolism. Proteins of the SSB family exclusively and transiently bind to ssDNA, preventing the DNA double helix from re-annealing and maintaining genome integrity. In the meantime,
Externí odkaz:
https://doaj.org/article/b2a3c94e566c4f559c3cd48ed3ecb0b5
Autor:
Daan Vorselen, Susan M. van Dommelen, Raya Sorkin, Melissa C. Piontek, Jürgen Schiller, Sander T. Döpp, Sander A. A. Kooijmans, Brigitte A. van Oirschot, Birgitta A. Versluijs, Marc B. Bierings, Richard van Wijk, Raymond M. Schiffelers, Gijs J. L. Wuite, Wouter H. Roos
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Red blood cell disorders are often accompanied by increased release of extracellular vesicles (EVs), but their structural and mechanical properties are not fully understood. Here, the authors show that red blood cell EVs show liposome-like mechanical
Externí odkaz:
https://doaj.org/article/ee3d649d089e45a59a0d4b1fccb687f2
Publikováno v:
Frontiers in Molecular Biosciences, Vol 7 (2020)
Both natural as well as artificial vesicles are of tremendous interest in biology and nanomedicine. Small vesicles (
Externí odkaz:
https://doaj.org/article/2104cf73f43e49fe8f55914d1b407d9f