Combined shRNA over CRISPR/cas9 as a methodology to detect off-target effects and a potential compensatory mechanism.

Autor: Peretz L; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Besser E; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Hajbi R; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Casden N; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Ziv D; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Kronenberg N; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Gigi LB; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Sweetat S; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Khawaled S; Lautenberg Center for Immunology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Aqeilan R; Lautenberg Center for Immunology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel., Behar O; Department of Developmental Biology and Cancer Research, The Institute for Medical Research, Faculty of Medicine, The Hebrew University, Ein Kerem, P.O. Box 12271, Jerusalem, 91120, Israel. odedb@ekmd.huji.ac.il.

Publikováno v: Scientific reports [Sci Rep] 2018 Jan 08; Vol. 8 (1), pp. 93. Date of Electronic Publication: 2018 Jan 08.