Addressing Critical Issues Related to Storage and Stability of the Vault Nanoparticle Expressed and Purified from Komagataella phaffi

Autor: Giulia Tomaino, Camilla Pantaleoni, Diletta Ami, Filomena Pellecchia, Annie Dutriaux, Linda Barbieri, Stefania Garbujo, Antonino Natalello, Paolo Tortora, Gianni Frascotti

Publikováno v: International Journal of Molecular Sciences
Volume 24
Issue 4
Pages: 4214

The vault nanoparticle is a eukaryotic assembly consisting of 78 copies of the 99-kDa major vault protein. They generate two cup-shaped symmetrical halves, which in vivo enclose protein and RNA molecules. Overall, this assembly is mainly involved in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f6da178dad891e8e11feed37dd12c0a
https://hdl.handle.net/10281/405935

Functionalization of colloidal nanoparticles with a discrete number of ligands based on a 'HALO-bioclick' reaction

Autor: Lucia Salvioni, Miriam Colombo, Xing Sun, Stefania Garbujo, Elisabetta Galbiati, Wolfgang J. Parak, Davide Prosperi, Matteo Mazzucchelli, Gianni Frascotti, Neus Feliu, Saad Megahed

A recombinant HALO-GFP fusion protein was designed and isolated to demonstrate the feasibility of controlling the number and orientation of protein ligands to be conjugated on colloidal gold nanoparticles. AuNPs functionalized with exactly one or exa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4ab3e1daab27f50178754c408721e62
http://hdl.handle.net/10281/288863

Engineered Ferritin Nanoparticles for the Bioluminescence Tracking of Nanodrug Delivery in Cancer

Autor: Laura Marongiu, Francesca Mingozzi, Lucia Salvioni, Veronica Tinelli, Luisa Fiandra, Michela Bellini, Michael S. Christodoulou, Miriam Colombo, Davide Prosperi, Alberto Visioli, Benedetta Riva, Maria Antonietta Rizzuto, Gianni Frascotti, Daniele Passarella

Publikováno v: Small. 16:2001450

The identification of a highly sensitive method to check the delivery of administered nanodrugs into the tumor cells is a crucial step of preclinical studies aimed to develop new nanoformulated cures, since it allows the real therapeutic potential of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc35612c3919d0605e5c3efb65e28b60
https://doi.org/10.1002/smll.202001450

Advances in molecular tools for the use of Zygosaccharomyces bailii as host for biotechnological productions and construction of the first auxotrophic mutant

Autor: Luca Riboldi, Davide Cattaneo, Minoska Valli, Gianni Frascotti, Danilo Porro, Simone Passolunghi, Laura Dato, Paola Branduardi

Publikováno v: FEMS Yeast Research. 10:894-908

The nonconventional yeast Zygosaccharomyces bailii has been proposed as a new host for biotechnological processes due to convenient properties such as its resistance to high sugar concentrations, relatively high temperatures and especially to acidic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9dd5650155446662f5e2e93b1754f039
https://doi.org/10.1111/j.1567-1364.2010.00668.x

Influence of growth temperature on the production of antibody Fab fragments in different microbes:A host comparative analysis

Autor: Lise Bernard-Granger, Jaana Tokkanen, Danilo Porro, Martin Dragosits, Gianni Frascotti, Tiina Pakula, Diethard Mattanovich, Kristin Baumann, Escarlata Rodríguez-Carmona, Michael Maurer, Maria Luisa Tutino, Renate Kunert, Paola Branduardi, Maria Giuliani, Merja Penttilä, Felícitas Vázquez, Michael Sauer, Pau Ferrer, Antonio Villaverde, Brigitte Gasser, Marilyn G. Wiebe, Ermenegilda Parrilli, Markku Saloheimo

Publikováno v: Dragosits, M, Frascotti, G, Bernard-Granger, L, Vázquez, F, Giuliani, M, Baumann, K, Rodríguez-Carmona, E, Tokkanen, J, Parrilli, E, Wiebe, M G, Kunert, R, Maurer, M, Gasser, B, Sauer, M, Branduardi, P, Pakula, T, Saloheimo, M, Penttilä, M, Ferrer, P, Luisa Tutino, M, Villaverde, A, Porro, D & Mattanovich, D 2011, ' Influence of growth temperature on the production of antibody Fab fragments in different microbes : A host comparative analysis ', Biotechnology Progress, vol. 27, no. 1, pp. 38-46 . https://doi.org/10.1002/btpr.524

Microorganisms encounter diverse stress conditions in their native habitats but also during fermentation processes, which have an impact on industrial process performance. These environmental stresses and the physiological reactions they trigger, inc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::407121f51f54eb62f63aa5eeaa7eb5c1
https://cris.vtt.fi/en/publications/396bb327-eec4-4ed5-a950-af3e7a4179d4