Protocol for the hyperinsulinemic euglycemic clamp to measure glucose kinetics in rats

Autor: Sandra Pereira, Margaret K. Hahn, Bailey Humber, Talha Chaudhry, Sally Wu, Sri Mahavir Agarwal, Nanka Dimitrova, Adria Giacca

Publikováno v: STAR Protocols, Vol 5, Iss 3, Pp 103143- (2024)

Summary: In rats, cannulation of the jugular vein and the carotid artery precedes the use of the hyperinsulinemic euglycemic clamp to determine insulin sensitivity in vivo. Here, we present a vascular surgery protocol to allow the infusion of substan

Externí odkaz: https://doaj.org/article/f454ba27157f479693eb92942c5c074e

EMID2 is a novel biotherapeutic for aggressive cancers identified by in vivo screening

Autor: Ambra Cappelletto, Edoardo Alfì, Nina Volf, Thi Van Anh Vu, Francesca Bortolotti, Giulio Ciucci, Simone Vodret, Marco Fantuz, Martina Perin, Andrea Colliva, Giacomo Rozzi, Matilde Rossi, Giulia Ruozi, Lorena Zentilin, Roman Vuerich, Daniele Borin, Romano Lapasin, Silvano Piazza, Mattia Chiesa, Daniela Lorizio, Luca Triboli, Sandeep Kumar, Gaia Morello, Claudio Tripodo, Maurizio Pinamonti, Giulia Maria Piperno, Federica Benvenuti, Alessandra Rustighi, Hanjoong Jo, Stefano Piccolo, Giannino Del Sal, Alessandro Carrer, Mauro Giacca, Serena Zacchigna

Publikováno v: Journal of Experimental & Clinical Cancer Research, Vol 43, Iss 1, Pp 1-14 (2024)

Abstract Background New drugs to tackle the next pathway or mutation fueling cancer are constantly proposed, but 97% of them are doomed to fail in clinical trials, largely because they are identified by cellular or in silico screens that cannot predi

Externí odkaz: https://doaj.org/article/a789156b8a0b44008d091dcfb970d332

Risk factors for emergency surgery for diverticulitis: A retrospective multicentric French study at 41 hospitals

Autor: Jeanne, Dembinski, Gillet, Julien, Hamy, Antoine, Lahmidi, Amine, Sebbagh, Mohamed, Bres, Antoine, Girard, Nicolas, Desjouis, Aurélie, Ainseba, Nadjib, Epailly, Julien, Huysman, Fabienne, Dezeustre, Maud, Doussot, Alexandre, Turco, Célia, Bourrhis, Diane, Godet, Camille, Finocchi, Morgane, Marion, Yoan, Lebreton, Gil, Benard, Flavie, Pineau, Elodie, Bouvier, Véronique, Ortega-Deballon, Pablo, Moreno-Lopez, Nathan, Rat, Patrick, Makar, Bassem, Fara, Paola, Pompilio, Marc, Hertault, Hugo, Guezennec, Corentin, Saida, Habchi, Brinza, Alexandre, Plard, Laurent, Ailhaud, Antoine, Gutierrez, Léa, Venchiarutti, Vincent, Faucheron, Jean-Luc, Morin, Bertrand, Pech de la Clause, Bertrand, Voitellier, Eglantine, Gagnat, Guillaume, Noiret, Barbara, Laroye, Constance, Piessen, Guillaume, Lécolle, Katia, Robert, Jean, Nzamushelepanmabla, Engel, Perrine, Mathonnet, Muriel, Rodriguez, Cyprien, Makki, Ammar, Couchard, Anne-Cécile, Duramé, François, Marceau, Cécile, Paquet, Jean-Christophe, Kepenekian, Vahan, Passot, Guillaume, Glehen ; Ninon Wallach, Olivier, Mohkam, Kayvan, Blanc, Marie-Caroline, Berdah, Stéphane, Aubert, Mathilde, Ayav, Ahmet, Chanty, Hervé, Didier, Alexandre, Vigneron, Estelle, Périard, Noémie, Thaumoux, Marie, Grenouilloux, Océane, Fall, Seila, Thomareis, Sophoclis, Marsollier, Chloé, Prud’homme, Michel, Antonopulos, Christos, Msika, Simon, Monsinjon, Marie, Giacca, Massimo, Cazelles, Antoine, Karoui, Mehdi, Malerba, Valentina, Dagher, Ibrahim, de Angelis, Nicola, Brunetti, Francesco, Ahmed, Omar, Ferraz, Jean-Marc, Ramjaun, Yassir, Denet, Christine, Fuchs, Basile, Pietrasz, Daniel, Raimbert, Pénélope, Benedetti, Juliette, Coton, Chloé, Corte, Hélène, Medrignac, Aude, Chavrier, Daphné, Girard, Robin, Berthelot, Julia, Monge, Matthieu, Papet, Eloïse, Pinson, Jean, Dolbeau, Elise, Tuech, Jean-Jacques, Chautard, Julien, Boullier, Mathilde, Goujard, François, Perrot, Laurent, Loge, Ludovic, Petiot, Jean-Michel, Manfredelli, Simone, Charleux-Muller, Diane, Sauvinet, Guillaume, Bertin, Jean-Baptiste, Vincent, Louis, Simeu, Blandine, Delhorme, Jean-Baptiste, Brigand, Cécile, Rohr, Serge, Bouchet, Manon, Duffas, Jean-Pierre, Ghouti, Laurent, Philis, Antoine, Portier, Guillaume, Karam, Elias, Michot, Nicolas, Artus, Alice, Mauny, Emilien, Bourbao-Tournois, Céline, Barbin, Hugo, Thiery, Julien, Sabbagh, Charles, Beyer-Berjot, Laura, Ouaissi, Mehdi, Zerbib, Philippe, Valérie, Bridoux, Manceau, Gilles, Panis, Yves, Buscail, Etienne, Venara, Aurélien, Khaoudy, Iman, Gaillard, Martin, Viennet, Manon, Thobie, Alexandre, Menahem, Benjamin, Eveno, Clarisse, Bonnel, Catherine, Mabrut, Jean-Yves, Badic, Bogdan, Eid, Yassine, Duchalais, Emilie, Lakkis, Zaher, Cotte, Eddy, Laforest, Anaïs, Desfourneaux-Denis, Véronique, Maggiori, Léon, Rebibo, Lionel, Niki, Christou, Talal, Ali, Mege, Diane, Bonnamy, Cécile, Germain, Adeline, Mauvais, François, Tresallet, Christophe, Roudie, Jean, Laurent, Alexis, Trilling, Bertrand, Bertrand, Martin, Massalou, Damien, Romain, Benoit, Tranchart, Hadrien, Pellegrin, Alexandra, Dejardin, Olivier, Alves, Arnaud

Publikováno v: In Surgery June 2024 175(6):1508-1517

Human blood vessel organoids reveal a critical role for CTGF in maintaining microvascular integrity

Autor: Sara G. Romeo, Ilaria Secco, Edoardo Schneider, Christina M. Reumiller, Celio X. C. Santos, Anna Zoccarato, Vishal Musale, Aman Pooni, Xiaoke Yin, Konstantinos Theofilatos, Silvia Cellone Trevelin, Lingfang Zeng, Giovanni E. Mann, Varun Pathak, Kevin Harkin, Alan W. Stitt, Reinhold J. Medina, Andriana Margariti, Manuel Mayr, Ajay M. Shah, Mauro Giacca, Anna Zampetaki

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-18 (2023)

Abstract The microvasculature plays a key role in tissue perfusion and exchange of gases and metabolites. In this study we use human blood vessel organoids (BVOs) as a model of the microvasculature. BVOs fully recapitulate key features of the human m

Externí odkaz: https://doaj.org/article/353954422197417aa51e7b271e88c1e2

Cell-Specific Effects of Insulin in a Murine Model of Restenosis Under Insulin-Sensitive and Insulin-Resistant Conditions

Autor: Marel Gonzalez Medina, Zhiwei Liu, Johny Wang, Cindy Zhang, Sarah B. Cash, Carolyn L. Cummins, Adria Giacca

Publikováno v: Cells, Vol 13, Iss 16, p 1387 (2024)

Restenosis following percutaneous revascularization is a major challenge in patients with insulin resistance and diabetes. Currently, the vascular effects of insulin are not fully understood. In vitro, insulin’s effects on endothelial cells (ECs) a

Externí odkaz: https://doaj.org/article/9bfe782017e04cfb9d4180f37729bb49