Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Gertrud Puu"'
Autor:
Gertrud Puu
Publikováno v:
Analytical Chemistry. 73:72-79
Biological membrane-like lipid films were deposited on the sensing surface in an optical biosensor instrument. The membranes were mixtures of biologically occurring lipids. Eight surfaces were prepared, some of which contained various glycolipids as
Publikováno v:
Biosensors and Bioelectronics. 15:31-41
Bacteriorhodopsin and the nicotinic acetylcholine receptor were biotinylated and reconstituted in lipidic membranes on silicon supports by fusion with proteoliposomes. The presence and distribution of the proteins were studied by binding with strepta
Publikováno v:
Journal of Microbiological Methods. 37:177-182
Helicobacter pylori, a human pathogen colonizing the gastrointestinal tract, is first interacting with mucus glycoproteins to penetrate the gastric mucus layer and then attach to specific epithelial cell targets. An optical biosensor technique based
Publikováno v:
Langmuir. 14:6705-6708
We have developed an electrode modification, which permits the communication between cytochrome c in solution, cytochrome oxidase residing in its natural environment of a lipid membrane, and a gold...
Autor:
Gertrud Puu, Torbjörn Tjärnhage
Publikováno v:
Colloids and Surfaces B: Biointerfaces. 8:39-50
A flow cell was designed for simultaneous mass measurements with a quartz crystal microbalance and ellipsometry and was used for studying the adsorption processes of different liposomes, prepared from phospholipids and cholesterol, on both bare and l
Publikováno v:
Bioelectrochemistry and Bioenergetics. 38:137-148
Stable lipid monolayers were deposited using the Langmuir-Blodgett technique onto silicon wafers, and were fused with liposomes containing acetylcholinesterase. The activity of the protein on the solid substrate was verified up to 28 days with a half
Publikováno v:
Biosensors and Bioelectronics. 10:463-476
Highly stable lipid bilayers, composed of biologically relevant lipids such as phosphatidylcholine, phospatidylethanolamine and cholesterol, were formed on platinum surfaces. Bacteriorhodopsin isolated from purple membrane (PM) from Halobacterium hal
Publikováno v:
Colloids and Surfaces B: Biointerfaces. 3:39-48
Autor:
Elisabet Artursson, Gertrud Puu
Publikováno v:
Canadian Journal of Microbiology. 38:1334-1337
An extracellular phosphatidylinositol-specific phospholipase C was isolated from a Cytophaga species. The growing conditions were optimized for the production of the enzyme and a purification scheme was developed. The enzyme is small and is not depen
Publikováno v:
Colloids and Surfaces. 64:275-287
Phosphatidic acid and a mixture of phosphatidic acid, phosphatidylcholine and cholesterol were transferred as an ordered monolayer to solid, hydrophilic supports by Langmuir—Blodgett technique. The supports were glass, chromium and platinum. Variab