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Robust estimation of bacterial cell count from optical density

Autor: Beal, J., Farny, G.N., Haddock-Angelli, T., Selvarajah, V., Baldwin, G.S., Buckley-Taylor, R., Gershater, M., Kiga, D., Marken, J., Sanchania, V., Sison, A., Workman, C.T., Crooijmans, M.E.

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______202::c381d0f8a21a7e9b01a3ebc87d4858c5
https://hdl.handle.net/1887/3629751

Reproducibility of fluorescent expression from engineered biological constructs in E. coli

Autor: Beal, J, Haddock-Angelli, T, Gershater, M, De Mora, K, Lizarazo, M, Hollenhorst, J, Rettberg, R, Demling, P, Hanke, R, Osthege, M, Schechtel, A, Sudarsan, S, Zimmermann, A, Gabryelczyk, B, Ikonen, M, Salmela, M, Acar, M, Aktas, MF, Bestepe, F, Ceylan, FS, Cigdem, S, Dohan, M, Elitok, M, Gunduz, M, Gunduz, E, Hatipoglu, OF, Kaya, T, Sayin, O, Tapan, S, Tereci, OF, Uçar, A, Yilmaz, M, Barrick, J, Gutierrez, A, Mishler, D, Monk, J, Mortensen, K, Shin, N, Watkins, E, Chen, Y, Jin, Y, Shi, Y, Zhang, HM, Ono, B, Paino, IMM, Ribovski, L, Silva, I, Zampronio, DK, Birkholz, N, Busche, RF, Konzock, O, Lippold, S, Ludwig, C, Philippi, M, Platz, L, Sigismund, C, Weber, S, Wehrs, M, Werchau, N, Wronska, A, Yen, ZZ, Agarwal, Y, Appleton, E, Densmore, D, Esmurria, A, Lewis, K, Pacheco, A, Bruchez, M, Peters, D, Telmer, C, Wang, L, Canas-Duarte, S, Giraldo-Perez, D, Gomez-Garzon, C, Madrid-Wolff, J, Marin-Medina, N, Mazzanti, V, Rodriguez-Forero, L, Scher, E, Dowell, R, O'Hara, S, Pogoda, CS, Shattuck, K, Altintas, A, Bali, AP, Bech, R, Egholm, A, Hansen, ASL, Jensen, K, Karlsen, KB, Mosbech, C, Belkhelfa, S, Berenger, N, Bodinier, R, Jacry, C

Publikováno v: Beal, J; Haddock-Angelli, T; Gershater, M; De Mora, K; Lizarazo, M; Hollenhorst, J; et al.(2016). Reproducibility of fluorescent expression from engineered biological constructs in E. coli. PLoS ONE, 11(3). doi: 10.1371/journal.pone.0150182. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/4h12628f

© 2016 Beal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cred

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::8a5ce8d74b99fce157e91812efb350f6
http://www.escholarship.org/uc/item/4h12628f

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