Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Gerry O'Beirne"'
Autor:
M I Bird, M Clifford, S M Game, Neil D. Cook, N E Nifant'ev, Gerry O'Beirne, N. V. Bovin, P K Rajapurohit, R. Priest
Publikováno v:
Analytical Biochemistry. 258:127-135
In this study, a novel scintillation proximity assay (SPA) that uses radiolabeled soluble neoglycoconjugates as synthetic alternatives to the natural E-, P-, and L-selectin counterligands was developed. The neoglycoconjugates contained sialyl LewisX
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c70edb07611c5c13a8c333aa76b1e23
https://doi.org/10.1006/abio.1998.2576
https://doi.org/10.1006/abio.1998.2576
Publikováno v:
Proceedings of the National Academy of Sciences. 94:11627-11632
The interactions between calmodulin, inositol 1,4,5-trisphosphate (Ins P 3 ), and pure cerebellar Ins P 3 receptors were characterized by using a scintillation proximity assay. In the absence of Ca 2+ , 125 I-labeled calmodulin reversibly bound to mu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ed67c324c23fd8f77693220519ee90a
https://doi.org/10.1073/pnas.94.21.11627
https://doi.org/10.1073/pnas.94.21.11627
Publikováno v:
Journal of Biochemical and Biophysical Methods. 34:177-187
We have developed an homogeneous assay to measure the uptake and efflux of [14C]adriamycin (doxorubicin hydrochloride) in human squamous lung carcinoma cells (SKMES-1), using 96 well scintillating microplates. The assay was also used to examine the e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7018f79949407c8cbcf9710979ed3f19
https://doi.org/10.1016/s0165-022x(97)01213-x
https://doi.org/10.1016/s0165-022x(97)01213-x
Publikováno v:
SLAS Discovery. 2:25-32
Activation of p34cdc2 requires a complex series of protein/protein interactions and specific phosphorylation and de-phosphorylation events to occur. The cellular role of p34cdc2 kinase lies in the regulation of eukaryotic cell entry into mitosis, in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::095ecd95b74bd6aa495e060240faa414
https://doi.org/10.1177/108705719700200106
https://doi.org/10.1177/108705719700200106
Publikováno v:
Cell calcium. 25(2)
Fura-2 and BAPTA were previously shown to be competitive antagonists of inositol trisphosphate (Ins P 3 ) receptors, but for practical reasons the analyses were performed at pH 8.3. We recently developed a scintillation proximity assay (SPA) for pure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d611d17a6810c30382aac530ab3befc
https://pubmed.ncbi.nlm.nih.gov/10326680
https://pubmed.ncbi.nlm.nih.gov/10326680
Publikováno v:
Biochemical and biophysical research communications. 221(3)
Inositol 1,4,5-triphosphate (Ins P 3 ) receptors are regulated by many intracellular signals including proteins and small messengers. By linking purified cerebellar Ins P 3 receptors to scintillation proximity assay beads, binding of radioligands can
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bc5c32d6717845ec4d107adde27c7b0
https://pubmed.ncbi.nlm.nih.gov/8630045
https://pubmed.ncbi.nlm.nih.gov/8630045