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Autor:
Gerrie A. F. Nicolaes, Arend J. Slotboom, Richard B. Lugtigheid, Hubertus M. Verheij, Edwin J.A. Veldhuizen, Gerard H. de Haas
Publikováno v:
European Journal of Biochemistry. 216:519-525
A porcine pancreatic phospholipase A2 mutant was constructed in which all nine lysines were replaced by arginines. The mutant displayed 68% residual activity on micellar zwitterionic substrates, indicating that lysines are not absolutely required for