Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Gernot Guigas"'
Publikováno v:
PLoS Computational Biology, Vol 7, Iss 6, p e1002067 (2011)
Using coarse-grained membrane simulations we show here that peripheral membrane proteins can form a multitude of higher-order structures due to membrane-mediated interactions. Peripheral membrane proteins characteristically perturb the lipid bilayer
Externí odkaz:
https://doaj.org/article/54379da551df41b0b67fe0a05c8e1529
Autor:
Peng Gao, G. Ulrich Nienhaus, Antonia F. Eckert, Gernot Guigas, Gabriela Matela, Karin Nienhaus
Publikováno v:
Chemical Communications. 53:979-982
Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED na
Autor:
Matthias Weiss, Gernot Guigas
Publikováno v:
Soft Matter. 11:33-37
Diffusion of membrane proteins is not only determined by the membrane anchor friction but also by the overall concentration of proteins and the length of their extra-membrane domains. We have studied the influence of the latter two cues by mesoscopic
Autor:
Andreas Veres, Gernot Guigas, Csilla Maria Ferencz, Matthias Weiss, Olaf Stemmann, Brigitte Neumann
Publikováno v:
Current Protocols in Cell Biology
Reconstitution of cellular organelles in vitro offers the possibility to perform quantitative and qualitative experiments in a controlled environment that cannot be done with the same accuracy in living cells. Following a previous report, the subsequ
Autor:
Dieter Trau, Matthias Weiss, Houwen Matthew Pan, Andreas Fery, Volker Patzel, Han Yu, Gernot Guigas
Publikováno v:
ACS applied materialsinterfaces. 9(6)
Hydrogels with complex internal structures are required for advanced drug delivery systems and tissue engineering or used as inks for 3D printing. However, hydrogels lack the tunability and diversity of polymeric shells and require complicated postsy
Autor:
Csilla Maria Ferencz, Andreas Veres, Olaf Stemmann, Matthias Weiss, Gernot Guigas, Brigitte Neumann
Publikováno v:
Biochimica et biophysica acta. 1858(9)
Organelles in eukaryotic cells often have complex shapes that deviate significantly from simple spheres. A prime example is the endoplasmic reticulum (ER) that forms an extensive network of membrane tubules in many mammalian cell types and in reconst
Autor:
Gernot Guigas, Matthias Weiss
Publikováno v:
Biophysical Journal. 94:90-94
Diffusion-mediated searching for interaction partners is an ubiquitous process in cell biology. Transcription factors, for example, search specific DNA sequences, signaling proteins aim at interacting with specific cofactors, and peripheral membrane
Publikováno v:
Biophysical Journal. 93:316-323
We have used fluorescence correlation spectroscopy to determine the anomalous diffusion properties of fluorescently tagged gold beads in the cytoplasm and the nucleus of living cells. From the extracted mean-square displacement v(tau) approximately t
Autor:
Gernot Guigas, Matthias Weiss
Publikováno v:
Biochimica et biophysica acta. 1858(10)
Cellular membranes are typically decorated with a plethora of embedded and adsorbed macromolecules, e.g. proteins, that participate in numerous vital processes. With typical surface densities of 30,000 proteins per μm(2) cellular membranes are indee
Autor:
Matthias Weiss, Gernot Guigas
Publikováno v:
Biophysical Journal. 91(7):2393-2398
Experimentally determined diffusion constants are often used to elucidate the size and oligomeric state of membrane proteins and domains. This approach critically relies on the knowledge of the size-dependence of diffusion. We have used mesoscopic si