Zobrazeno 1 - 10
of 438
pro vyhledávání: '"Germline transmission"'
Publikováno v:
BMC Veterinary Research, Vol 20, Iss 1, Pp 1-10 (2024)
Abstract Transgene silencing provides a significant challenge in animal model production via gene engineering using viral vectors or transposons. Selecting an appropriate strategy, contingent upon the species is crucial to circumvent transgene silenc
Externí odkaz:
https://doaj.org/article/3af1bedc1ff045e6aa064caa34e1d371
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Autor:
Mohammad Shamim Hossein, Young-Bum Son, Yeon Woo Jeong, Yeon Ik Jeong, Mi Na Kang, Eun Ji Choi, Kang Bae Park, Yu Ra Bae, Dae Young Kim, Woo Suk Hwang
Publikováno v:
Frontiers in Veterinary Science, Vol 10 (2023)
Propagation of transgenic animals by germline transmission using assisted reproductive technologies such as in vitro fertilization (IVF) is the most efficient way to produce transgenic colonies for biomedical research. The objective of this study was
Externí odkaz:
https://doaj.org/article/d759af5dcf984561be943bd57b866b52
Autor:
Jeffrey Lee, Jingjing Wang, Roxanne Ally, Sean Trzaska, Joseph Hickey, Alejo Mujica, Lawrence Miloscio, Jason Mastaitis, Brian Morse, Janell Smith, Amanda Atanasio, Eric Chiao, Henry Chen, Adrianna Latuszek, Ying Hu, David Valenzuela, Carmelo Romano, Brian Zambrowicz, Wojtek Auerbach
Publikováno v:
Stem Cell Reports, Vol 18, Iss 1, Pp 394-409 (2023)
Summary: Rats were more frequently used than mice to model human disease before mouse embryonic stem cells (mESCs) revolutionized genetic engineering in mice. Rat ESCs (rESCs) were first reported over 10 years ago, yet they are not as frequently used
Externí odkaz:
https://doaj.org/article/88b937e03131464dbeca15f69d7100e2
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Autor:
Jianxiang Lin, Xingchen Liu, Zongyang Lu, Shisheng Huang, Susu Wu, Wenxia Yu, Yao Liu, Xiaoguo Zheng, Xingxu Huang, Qiang Sun, Yunbo Qiao, Zhen Liu
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 25, Iss , Pp 494-501 (2021)
Prime editing enables efficient introduction of targeted transversions, insertions, and deletions in mammalian cells and several organisms. However, genetic disease models with base deletions by prime editing have not yet been reported in mice. Here,
Externí odkaz:
https://doaj.org/article/6660187d2dc3426d8bc611ea355af90e
Autor:
Soo-Ji Park, Tae Yeong Jeong, Seung Kyun Shin, Da Eun Yoon, Soo-Yeon Lim, Sol Pin Kim, Jungmin Choi, Hyunji Lee, Jeong-Im Hong, Jinhee Ahn, Je Kyung Seong, Kyoungmi Kim
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-11 (2021)
Abstract Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-edi
Externí odkaz:
https://doaj.org/article/090e19d9eb3b4d668ab587bfea39465f
Autor:
Bence Lázár, Mariann Molnár, Nikoletta Sztán, Barbara Végi, Árpád Drobnyák, Roland Tóth, Nikolett Tokodyné Szabadi, Michael J. McGrew, Elen Gócza, Eszter Patakiné Várkonyi
Publikováno v:
Poultry Science, Vol 100, Iss 8, Pp 101207- (2021)
ABSTRACT: Primordial germ cells (PGCs) are the precursors of germline cells that generate sperm and ova in adults. Thus, they are promising tools for gene editing and genetic preservation, especially in avian species. In this study, we established st
Externí odkaz:
https://doaj.org/article/d4852e0bd5294fc9b1f0280db2e2b5e6
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 9, Iss 1, Pp 287-295 (2019)
The CRISPR/Cas9 has been successfully applied for disruption of protein coding sequences in a variety of organisms. The majority of the animal genome is actually non-coding sequences, which are key regulators associated with various biological proces
Externí odkaz:
https://doaj.org/article/7a626a8ba1cb4352a3bb7cad4079b3b9
Autor:
Mashoodh, Rahia, Habrylo, Ireneusz B., Gudsnuk, Kathryn M., Pelle, Geralyn, Champagne, Frances A.
Publikováno v:
Proceedings: Biological Sciences, 2018 Mar . 285(1874), 1-10.
Externí odkaz:
https://www.jstor.org/stable/26545083