Elucidating sequence-function relationships in a template-independent polymerase to enable novel DNA recording applications.

Autor: Milisavljevic M; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois, USA., Rodriguez TR; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA., Tyo KEJ; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA.; Center for Synthetic Biology, Northwestern University, Evanston, Illinois, USA.

Publikováno v: Biotechnology and bioengineering [Biotechnol Bioeng] 2024 Dec; Vol. 121 (12), pp. 3808-3821. Date of Electronic Publication: 2024 Sep 14.

Substrate interactions guide cyclase engineering and lasso peptide diversification.

Autor: Barrett SE; Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA., Yin S; Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, Urbana, IL, USA., Jordan P; Lassogen, Inc., San Diego, CA, USA., Brunson JK; Lassogen, Inc., San Diego, CA, USA., Gordon-Nunez J; Lassogen, Inc., San Diego, CA, USA., Costa Machado da Cruz G; Lassogen, Inc., San Diego, CA, USA., Rosario C; Lassogen, Inc., San Diego, CA, USA., Okada BK; Lassogen, Inc., San Diego, CA, USA., Anderson K; Lassogen, Inc., San Diego, CA, USA., Pires TA; Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA., Wang R; Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA., Shukla D; Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA.; Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, Urbana, IL, USA.; Center for Biophysics and Quantitative Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA.; Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, IL, USA., Burk MJ; Lassogen, Inc., San Diego, CA, USA. mburk@lassogen.com., Mitchell DA; Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, IL, USA. douglasm@illinois.edu.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA. douglasm@illinois.edu.; Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, IL, USA. douglasm@illinois.edu.

Publikováno v: Nature chemical biology [Nat Chem Biol] 2024 Sep 11. Date of Electronic Publication: 2024 Sep 11.

An in-cluster Sfp-type phosphopantetheinyl transferase instead of the holo-ACP synthase activates the granaticin biosynthesis under natural physiological conditions.

Autor: Ming-Rong Deng¹ dengmr@gdim.cn, Sin Yu Chik¹, Yan Li¹, Honghui Zhu¹ zhuhh_gdim@163.com, Guohui Pan², Hui Jiang,³, Xudong Qu⁴

Publikováno v: Frontiers in Chemistry. 2024, p01-15. 15p.

Exploring the sequence-function space of microbial fucosidases.

Autor: Martínez Gascueña, Ana¹ (AUTHOR), Wu, Haiyang^1,2 (AUTHOR), Wang, Rui^3,4,5 (AUTHOR), Owen, C. David^6,7 (AUTHOR), Hernando, Pedro J.^1,8 (AUTHOR), Monaco, Serena⁹ (AUTHOR), Penner, Matthew^6,7 (AUTHOR), Xing, Ke⁵ (AUTHOR), Le Gall, Gwenaelle¹⁰ (AUTHOR), Gardner, Richard¹¹ (AUTHOR), Ndeh, Didier^1,12 (AUTHOR), Urbanowicz, Paulina A.¹¹ (AUTHOR), Spencer, Daniel I. R.¹¹ (AUTHOR), Walsh, Martin^6,7 (AUTHOR), Angulo, Jesus^9,13,14 (AUTHOR), Juge, Nathalie¹ (AUTHOR) nathalie.juge@quadram.ac.uk

Publikováno v: Communications Chemistry. 6/18/2024, Vol. 7 Issue 1, p1-15. 15p.

Cytochromes P450 involved in bacterial RiPP biosyntheses.

Autor: Kunakom, Sylvia¹ (AUTHOR), Otani, Hiroshi^1,2 (AUTHOR), Udwary, Daniel W^1,2 (AUTHOR), Doering, Drew T¹ (AUTHOR), Mouncey, Nigel J^1,2 (AUTHOR) nmouncey@lbl.gov

Publikováno v: Journal of Industrial Microbiology & Biotechnology. Dec2023, Vol. 50 Issue 1, p1-14. 14p.