Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Gerhard Steinborn"'
Publikováno v:
FEMS Yeast Research. 7:1181-1187
A wide-range yeast vector (CoMed) system has been applied to the comparative assessment of three different yeast platforms for the production of human interleukin-6. A vector equipped with an rRNA gene targeting sequence and an Arxula adeninivorans-d
APHO1 from the yeast Arxula adeninivorans encodes an acid phosphatase of broad substrate specificity
Autor:
Gotthard Kunze, Jelena Põlajeva, Parvinder Kaur, Erik Böer, Tulasi Satyanarayana, Gerhard Steinborn, Gerd Gellissen, Anja Lingner, Bijinder Singh, Rüdiger Bode
Publikováno v:
Antonie van Leeuwenhoek. 91:45-55
The extracellular acid phosphatase-encoding Arxula adeninivorans APHO1 gene was isolated using degenerated specific oligonucleotide primers in a PCR screening approach. The gene harbours an ORF of 1449 bp encoding a protein of 483 amino acids with a
Publikováno v:
FEMS Yeast Research. 5:1047-1054
Different targeting sequences derived from the Arxula adeninivorans and Hansenula polymorpha rDNA clusters were tested in A. adeninivorans integration/expression vectors. For element identification, the rDNA unit of A. adeninivorans (accession number
Publikováno v:
Yeast Biotechnology: Diversity and Applications ISBN: 9781402082917
No single yeast-based platform exists which is optimal for every protein. It is advisable to assess several platform candidates in parallel for optimal expres- sion characteristics in a given case. For this approach, a wide-range yeast vector has bee
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::da6edc8600d2d598c53f564c8e3d5af3
https://doi.org/10.1007/978-1-4020-8292-4_17
https://doi.org/10.1007/978-1-4020-8292-4_17
Publikováno v:
FEMS yeast research. 7(7)
An Arxula adeninivorans vector element has been identified that provides multicopy integration in an atrp1 host strain. The element consists of the ATRP1 selection marker fused to a newly generated truncated ALEU2 promoter of 53 bp. In the described
Publikováno v:
Applied microbiology and biotechnology. 77(3)
Yeasts provide attractive expression platforms. They combine ease of genetic manipulations and the option for a simple fermentation design of a microbial organism with the capabilities of an eukaryotic organism to secrete and to modify a protein acco
Publikováno v:
Microbial Cell Factories, Vol 5, Iss 1, p 33 (2006)
Microbial Cell Factories
Microbial Cell Factories
Background Yeasts provide attractive expression platforms in combining ease of genetic manipulation and fermentation of a microbial organism with the capability to secrete and to modify proteins according to a general eukaryotic scheme. However, earl
Publikováno v:
Journal of biotechnology. 127(3)
A host/vector expression system based on an Arxula adeninivorans Delta atrp1 gene disruption mutant has been constructed. For this purpose the ATRP1 gene encoding a phosphoribosyl anthranilate isomerase was isolated from the yeast A. adeninivorans an
Publikováno v:
FEMS yeast research. 5(11)
Different targeting sequences derived from the Arxula adeninivorans and Hansenula polymorpha rDNA clusters were tested in A. adeninivorans integration/expression vectors. For element identification, the rDNA unit of A. adeninivorans (accession number
Publikováno v:
Archives of microbiology. 183(2)
The genes encoding the biosynthesis of the dipeptide bacilysin and its antibiotic constituent anticapsin were isolated from several strains of Bacillus subtilis as well as B. amyloliquefaciens and B. pumilus. The ywfBCDEF genes of B. subtilis 168 wer