Retracted Article: Microelectrochemical cell arrays for whole-cell currents recording through ion channel proteins based on trans-electroporation approach

Autor: Tianyang Zheng, Gerhard Baaken, Jan C. Behrends, Jürgen Rühe

Publikováno v: The Analyst. 145:197-205

A novel chip based method is able to record the whole-cell currents through biological cell membrane with high electrostability.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::87767cfe5d7f40da020107ee938f9fe4
https://doi.org/10.1039/c9an01737b

Pore-forming toxins as tools for polymer analytics: From sizing to sequencing

Autor: Gerhard Baaken, Ekaterina Zaitseva, Manuela Pastoriza-Gallego, Jan C. Behrends, Hadjer Ouldali, Tobias Ensslen, Mazdak Afshar Bakshloo, Fabien Piguet, Monasadat Talarimoghari, Abdelghani Oukhaled

Publikováno v: Methods in Enzymology ISBN: 9780128238585
Pore-Forming Toxins
Pore-Forming Toxins, 649, Elsevier, pp.587-634, 2021, Methods in Enzymology, ⟨10.1016/bs.mie.2021.01.017⟩

We report here on the nanopore resistive pulse sensing (Np-RPS) method, involving pore-forming toxins as tools for polymer analytics at single molecule level. Np-RPS is an electrical method for the label-free detection of single molecules. A molecule

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7d28d4145e6ee2ebff1e46f95fa96a6
https://doi.org/10.1016/bs.mie.2021.01.017

Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers: Next-Generation Instrumentation for Multiple Recordings in Parallel

Autor: Ekaterina, Zaitseva, Alison, Obergrussberger, Conrad, Weichbrodt, Mordjane, Boukhet, Frank, Bernhard, Christopher, Hein, Gerhard, Baaken, Niels, Fertig, Jan C, Behrends

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2188

Artificial lipid bilayers have been used for several decades to study channel-forming pores and ion channels in membranes. Until recently, the classical two-chamber setups have been primarily used for studying the biophysical properties of pore formi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::94b9e971fdd8518a72b125952d591e1f
https://pubmed.ncbi.nlm.nih.gov/33119847

Validation of ADAM10 metalloprotease as aBacillus thuringiensisCry3Aa toxin functional receptor in Colorado potato beetle (Leptinotarsa decemlineata)

Autor: Gerhard Baaken, Victor M. Ruiz-Arroyo, Ekaterina Zaitseva, Galo A. Goig, Amparo C. Martínez-Ramírez, Inmaculada García-Robles, María Dolores Real, Carolina Rausell, Camila Ochoa-Campuzano

Publikováno v: Insect Molecular Biology. 26:204-214

Bacillus thuringiensis parasporal crystal proteins (Cry proteins) are insecticidal pore-forming toxins that bind to specific receptor molecules on the brush border membrane of susceptible insect midgut cells to exert their toxic action. In the Colora

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::64495142b15a910daa1a1403b145b33f
https://doi.org/10.1111/imb.12285

Activity of the Gramicidin A Ion Channel in a Lipid Membrane with Switchable Physical Properties

Autor: Ibrahim Halimeh, Jan C. Behrends, Renate Reiter, Andreas Zumbuehl, Gerhard Baaken, Ekaterina Zaitseva

Publikováno v: Langmuir : the ACS journal of surfaces and colloids. 35(46)

Lipid bilayer membranes formed from the artificial 1,3-diamidophospholipid Pad-PC-Pad have the remarkable property that their hydrophobic thickness can be modified in situ: the particular arrangement of the fatty acid chains in Pad-PC-Pad allows them

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::997376634b452309d4ee59fcb9813de4
https://pubmed.ncbi.nlm.nih.gov/31645105