Zobrazeno 1 - 10
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pro vyhledávání: '"Gerard Peaucellier"'
Publikováno v:
Development, Growth and Differentiation. 35:199-208
The egg plasma membrane and cortical structures are highly enriched in protein tyrosine kinase activity which is thought to play an important role in the fertilization process. In order to identify the tyrosine protein kinases in the egg cortex, a si
Publikováno v:
Developmental biology. 146(1)
Fertilization of the echinoderm egg is known to result in the phosphorylation, on tyrosine, of a high-molecular-weight cortical protein (HMWCP) localized in the egg cortex. Studies using various parthenogenic agents indicate that this phosphorylation
Publikováno v:
Developmental biology. 138(2)
We have used an antibody specific for phosphotyrosine to investigate protein phosphorylation on tyrosine during hormone-induced maturation of starfish oocytes. Analysis of immunoprecipitates from cortices of in vivo labeled Marthasterias glacialis oo
Publikováno v:
Development, Growth and Differentiation. 27:251-262
Single nuclei (germinal vesicles) manually isolated from large oocytes of the starfish Echinaster sepositus, as well as the complementary anucleated oocytes, were used to investigate the early changes of protein phosphorylation which occur from 1-MeA
Publikováno v:
Gamete Research. 5:115-123
Incorporation of [32P]-phosphate into proteins was enhanced when Sabellaria oocytes were stimulated with specific protease to continue from prophase I block to metaphase I block. The rate of incorporation was increased 50 fold between onset of treatm
Autor:
Christian Le Peuch, Françoise Le Bouffant, Gerard Peaucellier, Jean-Claude Labbé, André Picard, Marcel Dorée
Publikováno v:
Development, Growth and Differentiation. 29:93-103
Many studies suggest that MPF activation depends on protein phosphorylation or that MPF is itself a protein kinase. In the present report, cyclic variations of MPF activity have been correlated in vivo with changes in the extent of protein phosphoryl
Autor:
Gerard Peaucellier, Marcel Dorée
Publikováno v:
Development, Growth and Differentiation. 23:287-296
Fertilization or activation by ionophore A 23187 induces a transient acid release in prophase-blocked and in maturing oocytes of Asterias rubens and Marthasterias glacialis. 1-Methyladenine-induced maturation is not accompanied by acid release. There
Publikováno v:
Journal of Biological Chemistry. 263:13806-13811
The sea urchin egg contains one or more protein tyrosine kinases which are active during the response of the egg to fertilization. In the present study, we have used an antibody specific for phosphotyrosine to determine which egg proteins are phospho
Publikováno v:
Development, Growth and Differentiation. 31:573-580
Fertilization of the sea urchin egg is accompanied by activation of one or more protein tyrosine kinases which have been shown to phosphorylate a restricted set of egg proteins in vitro. In order to characterize these tyrosine kinase substrates, we h
Publikováno v:
Developmental biology. 109(2)
In starfish oocytes, activity of the maturation-promoting factor (MPF) and that of a major cAMP-independent protein kinase dropped at the time of meiotic cleavage, and rose again after the first but not the second meiotic cleavage. Protein synthesis