Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Gerald W. Stemke"'
Autor:
Walter A. Blank, Gerald W. Stemke
Publikováno v:
Canadian Journal of Microbiology. 46:832-840
Autor:
Janet A. Robertson, Gerald W. Stemke
Publikováno v:
Veterinary Microbiology. 24:135-142
Cultures of Mycoplasma hyopneumoniae and M. flocculare in Friis' broth grew faster and to higher titers in air than in 8% CO2; cultures in air grew better when shaken than when stationary. Under the optimal conditions, both species have generation ti
Publikováno v:
Nucleic Acids Research. 18:1451-1455
Contour clamped homogeneous field (CHEF) agarose gel electrophoresis (AGE), ramped to give linear separation of DNA molecules of 600-1600 kilobase pairs (kbp), was used to determine mobilities for full-sized genomic DNA of the serotype standard strai
Publikováno v:
International Journal of Systematic Bacteriology. 44:836-838
Two-by-two sequence alignment revealed that the levels of homology between 16S rRNA gene sequences of strains belonging to the two biovars of Ureaplasma urealyticum (class Mollicutes) ranged from 98.5 to 98.9%. Within the biovars, three serovars of t
Autor:
Janet A. Robertson, Gerald W. Stemke, David Thirkell, Maurice C. Shepard, Ryĵ Harasawa, Fanrong Kong, Denys K Ford, John W Davis
Publikováno v:
International journal of systematic and evolutionary microbiology. 52(Pt 2)
The phenotypic and genotypic properties of Ureaplasma urealyticum (family Mycoplasmataceae, order Mycoplasmatales, class Mollicutes) are reviewed here. The 14 recognized serovar standard strains found in humans exhibit no serological cross-reactivity
Autor:
Gerald W. Stemke, Walter A. Blank
Publikováno v:
Canadian journal of microbiology. 46(9)
A macrorestriction map of the genome of Mycoplasma hyopneumoniae strain J, the type strain of the causative agent of enzootic pneumonia in pigs, was constructed using pulsed-field gel electrophoresis (PFGE) and DNA hybridization. The size of the geno
Publikováno v:
International journal of systematic bacteriology. 46(4)
The phylogenetic positions of the porcine mycoplasmas Mycoplasma hyosynoviae and Mycoplasma hyopharyngis were determined by using PCR-amplified 16S rRNA gene sequences. M. hyosynoviae is a member of the Mycoplasma hominis group, while M. hyopharyngis
Autor:
Janet A. Robertson, Gerald W. Stemke
Publikováno v:
International journal of systematic bacteriology. 46(4)
Ureaplasma gallorale is a urease-containing mycoplasma (a member of the Mollicutes) which is pathogenic for chickens, from which it was originally isolated. We amplified the 16S rRNA gene of this bacterium and then cloned and sequenced the amplicon.
Autor:
Richard F. Ross, Gerald W. Stemke
This chapter presents the diagnostic techniques for swine mycoplasma infections. Methods for isolation of M. hyopneumoniae , given in detail in Ross and Whittlestone, included media formulations, selective procedures for isolation of the organism in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97975465f44c1dd17b914c383349ff10
https://doi.org/10.1016/b978-012583806-1/50032-8
https://doi.org/10.1016/b978-012583806-1/50032-8
Autor:
Janet A. Robertson, Gerald W. Stemke
Publikováno v:
Molecular and Diagnostic Procedures in Mycoplasmology
This chapter provides an overview of measurement of mollicute growth by adenosine triphosphate (ATP)-dependent luminometry. Mollicute cells have notably smaller cellular mass and their cultures produce smaller populations than do other free-living pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f3fd738c38ae2d179b21156b9c51739a
https://doi.org/10.1016/b978-012583805-4/50008-x
https://doi.org/10.1016/b978-012583805-4/50008-x