Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Gerald R. Kovacs"'
Autor:
Seema S Gangolli, Nikos Vasilakis, Darlene Falvey, Timothy J. Zamb, Stephen A. Udem, Jacek Kowalski, Gerald R. Kovacs, John W. Coleman
Publikováno v:
Nature Biotechnology. 21:932-935
This report describes a transfection-independent system for packaging alphavirus replicon vectors using modified vaccinia virus Ankara (MVA) vectors to express all of the RNA components necessary for the production of Venezuelan equine encephalitis (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a9bb3f7a284948b1440e015219d5479
https://doi.org/10.1038/nbt845
https://doi.org/10.1038/nbt845
Publikováno v:
Journal of Virological Methods. 111:29-36
A modified cDNA rescue system that improves recovery of recombinant nonsegmented, negative-strand RNA viruses from cloned DNAs is described. Rescue systems based on vaccinia virus-T7 RNA polymerase vectors have been used to derive many negative-stran
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f6b91c10fd6f7882f3496fab44a93c0
https://doi.org/10.1016/s0166-0934(03)00132-0
https://doi.org/10.1016/s0166-0934(03)00132-0
Publikováno v:
Journal of Virological Methods. 109:133-138
Alphavirus replicon particles are being exploited for a variety of purposes both in vitro as gene expression vectors, and in vivo as vaccines or gene therapy vectors. There is a need for a simple and universal method of titration of replicon particle
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccd9a9c717c31ddca3cbcf0f89e81e18
https://doi.org/10.1016/s0166-0934(03)00063-6
https://doi.org/10.1016/s0166-0934(03)00063-6
Publikováno v:
Journal of Virology. 70:4444-4450
Evidence is presented that a 26-kDa protein encoded by the vaccinia virus A2L open reading frame, originally shown to be one of three intermediate-stage genes that together can transactivate late-stage gene expression in transfection assays (J. G. Ke
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7892a19f8a7698b8b734c8d2d5058e2
https://doi.org/10.1128/jvi.70.7.4444-4450.1996
https://doi.org/10.1128/jvi.70.7.4444-4450.1996
Autor:
Ronald G. Manning, Richard Hatchett, Joanna M. Prasher, Gary L. Disbrow, Kenneth D. Cliffer, Gerald R. Kovacs, Judith L. Bader, C. Norman Coleman, Marcy B. Grace, Brian R. Moyer, John R. Mercier
Publikováno v:
Health physics. 101(3)
Following the attacks of 11 September 2001, emergency preparedness within the U.S. Department of Health and Human Services, as well as at the Department of Defense and other federal agencies, received higher visibility, new mandates and increased fun
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::522cd56d261ce0fa4f6a3a7357722e92
https://pubmed.ncbi.nlm.nih.gov/21799340
https://pubmed.ncbi.nlm.nih.gov/21799340
Publikováno v:
Journal of Virology. 67:5740-5748
The A1L, A2L, and G8R open reading frames (ORFs) were previously shown by transfection assays to encode transactivators of late gene expression. We now present evidence that the 17-kDa protein product of the A1L gene can function in vitro as a transc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d7f8787b141222ef9dba00b34e7a521
https://doi.org/10.1128/jvi.67.10.5740-5748.1993
https://doi.org/10.1128/jvi.67.10.5740-5748.1993
Publikováno v:
Virology. 185:633-643
Previous to this study, the Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) was known to express only one spliced RNA (spliced IE1 or IE0). We have conducted an analysis of RNA expressed during infection of Spodoptera frugiperd
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd0f8381602a96dfb55518abce198ac3
https://doi.org/10.1016/0042-6822(91)90534-i
https://doi.org/10.1016/0042-6822(91)90534-i
Autor:
Miles W. Carroll, Gerald R. Kovacs
Publikováno v:
Gene Transfer and Expression in Mammalian Cells ISBN: 9780444513717
Publisher Summary The chapter presents basic concepts and applications of poxviruses as recombinant expression vectors. Additional focused reviews on poxvirus molecular biology construction of recombinant poxviruses and their applications as vaccine
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a401efcc4201cc22ad6677d09d4ed715
https://doi.org/10.1016/s0167-7306(03)38008-1
https://doi.org/10.1016/s0167-7306(03)38008-1
Autor:
Christopher L. Parks, Stephen A. Udem, Gerald R. Kovacs, Robert A. Lerch, Rebecca M. Nowak, Hai Ping Wang, Pramila Walpita, Nikos Vasilakis, Jacek Kowalski, Mohinderjit S. Sidhu
Publikováno v:
Virus research. 83(1-2)
A canine distemper virus (CDV) genomic cDNA clone and expression plasmids required to establish a CDV rescue system were generated from a laboratory-adapted strain of the Onderstepoort vaccine virus. In addition, a CDV minireplicon was prepared and u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6acddbb49294fd9e03b79015a4021d95
https://pubmed.ncbi.nlm.nih.gov/11864746
https://pubmed.ncbi.nlm.nih.gov/11864746
The B1 gene of vaccinia virus encodes a serine/threonine protein kinase that is expressed early after infection. Under nonpermissive conditions, temperature-sensitive mutants ( ts 2 and ts 25) that map to B1 fail to efficiently replicate viral DNA. O
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f711b6ad21cd3dd9483ea186ac1cb89e
https://europepmc.org/articles/PMC114150/
https://europepmc.org/articles/PMC114150/