Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Gerald P. Holmquist"'
Publikováno v:
Nucleic Acids Research
We show that the cAMP receptor protein (Crp) binds to DNA as several different conformers. This situation has precluded discovering a high correlation between any sequence property and binding affinity for proteins that bend DNA. Experimentally quant
Publikováno v:
Analytical Biochemistry. 310:179-185
We coupled ligation with mass action to achieve high-efficiency clamp attachment without polymerase chain reaction (PCR). Using a 10-fold molar excess of a GC-rich clamp of synthesized and hybridized oligonucleotides, we achieved the maximum clamp-li
Publikováno v:
Biochemistry. 40:12465-12471
Oxidative damage to DNA bases in isolated HeLa nuclei occurs upon treatment with rhodium intercalators and photoactivation. Oxidation occurs preferentially at the 5'-guanine of 5'-GG-3' sites, indicative of base damage by DNA-mediated charge transfer
Autor:
Susan P. LeDoux, Gerald P. Holmquist, Steven A. Akman, Wesley J. Driggers, Henry Rodriguez, Glenn L. Wilson
Publikováno v:
Methods. 22:148-156
Reactive oxygen species induce a pharmacopoeia of oxidized bases in DNA. DNA can be cleaved at most of the sites of these modified bases by digestion with a combination of two base excision repair glycosylases from Escherichia coli, Fpg glycosylase,
Publikováno v:
Biochemistry. 38:16578-16588
We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl
Publikováno v:
Journal of Molecular Biology. 284:269-285
Base excision repair rates of dimethyl sulfate-induced 3-methyladenine and 7-methylguanine adducts were measured at nucleotide resolution along the PGK1 gene in normal human fibroblasts. Rates of 7-methylguanine repair showed a 30-fold dependence on
Autor:
Nicole Lemieux, Patrick Scott, Gerald P. Holmquist, Raouf Fetni, Mohamed Boutouil, Claude-Lise Richer, Régen Drouin
Publikováno v:
Chromosoma. 106:405-411
Imprinted autosomal loci apparently reside in very large chromosomal domains that exhibit asynchrony in replication of homologous alleles during the DNA synthesis phase. Replication asynchrony can be cytogenetically visualized by a replication-bandin
Autor:
Gerald P. Holmquist, Gerd P. Pfeifer
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer. 1333:M1-M8
Autor:
Gerald P. Holmquist, Shuwei Gao
Publikováno v:
Mutation Research/Reviews in Mutation Research. 386:69-101
The theory of somatic mutagenesis predicts that the frequency pattern of induced selectable mutations along a gene is the product of the probability patterns of the several sequential steps of mutagenesis, e.g., damage, repair, polymerase misreading,
Autor:
Gerald P. Holmquist, Jan Filipski
Publikováno v:
Trends in Ecology & Evolution. 9:65-69
Recent advances in molecular mutagenesis reveal that two of the mechanisms which contribute to mutagen-induced point mutations, the frequency of induced DNA damage and the repair rate of this damage, vary considerably along the genome. At a grosser l