Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Gerald M. Adair"'
Autor:
Jennifer J. Rahn, Rodney S. Nairn, Megan P. Lowery, Gerald M. Adair, Brian Rowley, Luis Della Coletta, Tiffany Limanni
Publikováno v:
DNA Repair. 10:188-198
The ERCC1-XPF structure-specific endonuclease is necessary for correct processing of homologous recombination intermediates requiring the removal of end-blocking nonhomologies. We previously showed that targeting the endogenous CHO APRT locus with pl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fa955935a7c36192e0e55aca34abf0f
https://doi.org/10.1016/j.dnarep.2010.10.011
https://doi.org/10.1016/j.dnarep.2010.10.011
Autor:
Gerald M. Adair, Leisa L. Talbert, Angela Bolt, David Trono, Megan G. Lowery, Rodney S. Nairn, Luis Della Coletta
Publikováno v:
DNA Repair. 7:1319-1329
The UV hypersensitive CHO cell mutant UV41 is the archetypal XPF mammalian cell mutant, and was essential for cloning the human nucleotide excision repair (NER) gene XPF by DNA transfection and rescue. The ERCC1 and XPF genes encode proteins that for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2efacb304b53d2d9526ee3cc80487c82
https://doi.org/10.1016/j.dnarep.2008.04.012
https://doi.org/10.1016/j.dnarep.2008.04.012
Publikováno v:
Journal of Biological Chemistry. 276:16786-16796
Both prokaryotic and eukaryotic cells have the capacity to repair DNA damage preferentially in the transcribed strand of actively expressed genes. However, we have found that several types of DNA damage, including cyclobutane pyrimidine dimers (CPDs)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1aa17032ed4e751cfe6dcd7a3e7ec11a
https://doi.org/10.1074/jbc.m010973200
https://doi.org/10.1074/jbc.m010973200
Autor:
Gerald M. Adair, R. Geoffrey Sargent, Rodney S. Nairn, James L. Meservy, Brian D. Perkins, Zsofia Intody, John H. Wilson, April E. Kilburn
Publikováno v:
Nucleic Acids Research. 28:3771-3778
Spontaneous recombination between direct repeats at the adenine phosphoribosyltransferase (APRT) locus in ERCC1-deficient cells generates a high frequency of rearrangements that are dependent on the process of homologous recombination, suggesting tha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0351fc9bc89559521a66709a3a588a93
https://doi.org/10.1093/nar/28.19.3771
https://doi.org/10.1093/nar/28.19.3771
Publikováno v:
Mutagenesis. 13:357-365
We isolated and characterized the ERCC1 coding sequence from three Chinese hamster ovary (CHO) parental (CHO-AA8, CHO-AT3-2 and CHO-9) and 10 ERCC1 mutant cell lines. Two general classes of mutations were observed: two mutant cell lines exhibited nuc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65ba2b4a7fd02dc6ab4728e89d6479bd
https://doi.org/10.1093/mutage/13.4.357
https://doi.org/10.1093/mutage/13.4.357
Autor:
Gerald M. Adair, John H. Wilson, Rhonda L. Rolig, R. Geoffrey Sargent, April E. Kilburn, Rodney S. Nairn
Publikováno v:
Proceedings of the National Academy of Sciences. 94:13122-13127
Nucleotide excision repair proteins have been implicated in genetic recombination by experiments in Saccharomyces cerevisiae and Drosophila melanogaster , but their role, if any, in mammalian cells is undefined. To investigate the role of the nucleot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b62bea9cc3c0729615096e03561f008e
https://doi.org/10.1073/pnas.94.24.13122
https://doi.org/10.1073/pnas.94.24.13122
Publikováno v:
Nucleic Acids Research. 24:746-753
Several DNA sequence elements are thought to stimulate homologous recombination, illegitimate recombination, or both in mammalian cells. Some are implicated by their recurrence around rearrangement breakpoints, others by their effects on recombinatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1db052babafebb5c00865722ec43c5ba
https://doi.org/10.1093/nar/24.4.746
https://doi.org/10.1093/nar/24.4.746
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 920
The study of gene function has been greatly facilitated by the development of strategies to modify genomic DNA. Gene targeting is one of the most successfully applied techniques used to examine the roles of specific genes in a wide variety of model s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7ac8f6a3955ab9cbf7aed35d7ade7b37
https://pubmed.ncbi.nlm.nih.gov/22941622
https://pubmed.ncbi.nlm.nih.gov/22941622
Publikováno v:
Methods in Molecular Biology ISBN: 9781617799976
Gene targeting by homologous recombination in mammalian cells is an important tool for generating genetically modified mice used for modeling human diseases. Gene targeting approaches are also useful for studying the mechanisms of homologous recombin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4781083047f34e4f9d1541826c3bcfce
https://doi.org/10.1007/978-1-61779-998-3_31
https://doi.org/10.1007/978-1-61779-998-3_31
Autor:
Rodney S. Nairn, Michael Seidman, Debra G. Smith, Gerald M. Adair, John H. Wilson, Thomas W. Porter, Sandra L. Pennington
Publikováno v:
Somatic Cell and Molecular Genetics. 19:363-375
A 21-bp deletion in the third exon of the APRT gene in Chinese hamster ovary (CHO) cells was corrected by transfection with a plasmid containing hamster APRT sequences. Targeted correction frequencies in the range of 0.3-3.0 x 10(-6) were obtained wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4729f44447aeb62e83186d2a419d8d3
https://doi.org/10.1007/bf01232748
https://doi.org/10.1007/bf01232748