Výsledky vyhledávání - "Georgios Pothoulakis"

Akademický článek

Construction of hybrid regulated mother-specific yeast promoters for inducible differential gene expression.

Publikováno v: PLoS ONE, Vol 13, Iss 3, p e0194588 (2018)

Engineered promoters with predefined regulation are a key tool for synthetic biology that enable expression on demand and provide the logic for genetic circuits. To expand the availability of synthetic biology tools for S. cerevisiae yeast, we here u

Externí odkaz: https://doaj.org/article/e5b50e074ab24dd4b5de9e96c6a266d1

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Enzymatic assembly of small synthetic genes with repetitive elements

Autor: Michael T. A. Nguyen, Martin Vincent Gobry, Néstor Sampedro Vallina, Georgios Pothoulakis, Ebbe Sloth Andersen

Although gene synthesis efficiency has improved through the years, current methods offered by DNA synthesis vendors are limited when it comes to repetitive sequences. Here, we describe a method for the enzymatic assembly of repetitive small synthetic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::27dea4fd44ca1e2ebf318e32ac486779
https://doi.org/10.1101/2022.11.23.517630

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Utilizing RNA origami scaffolds in Saccharomyces cerevisiae for dCas9-mediated transcriptional control

Autor: Ebbe Sloth Andersen, Michael Truong-Giang Nguyen, Georgios Pothoulakis

Publikováno v: Pothoulakis, G, Nguyen, M T A & Andersen, E S 2022, ' Utilizing RNA origami scaffolds in Saccharomyces cerevisiae for dCas9-mediated transcriptional control ', Nucleic Acids Research, vol. 50, no. 12, pp. 7176-7187 . https://doi.org/10.1093/nar/gkac470

Designer RNA scaffolds constitute a promising tool for synthetic biology, as they can be genetically expressed to perform specific functions in vivo such as scaffolding enzymatic cascades and regulating gene expression through CRISPR-dCas9 applicatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::257530314992a217de992161d11d4cf3
https://pubmed.ncbi.nlm.nih.gov/35648481

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Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds

Autor: Ebbe Sloth Andersen, Michael Truong-Giang Nguyen, Georgios Pothoulakis

Publikováno v: Nguyen, M T A, Pothoulakis, G & Andersen, E S 2021, ' Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds ', ACS Synthetic Biology . https://doi.org/10.1021/acssynbio.1c00608
Nguyen, M T A, Pothoulakis, G & Andersen, E S 2022, ' Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds ', A C S Synthetic Biology, vol. 11, no. 5 . https://doi.org/10.1021/acssynbio.1c00608

Rational design approaches for the regulation of gene expression are expanding the synthetic biology toolbox. However, only a few tools for regulating gene expression at the translational level have been developed. Here, we devise an approach for tra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2e28310e668581ff306d23a0442dbde
https://pure.au.dk/portal/da/publications/synthetic-translational-regulation-by-proteinbinding-rna-origami-scaffolds(bdbcb241-1709-41cb-bb15-3a1ea7dc3c31).html

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Synthetic gene regulation for independent external induction of the

Autor: Georgios, Pothoulakis, Tom, Ellis

Publikováno v: Communications biology. 1

Pseudohyphal growth is a multicellular phenotype naturally performed by wild budding yeast cells in response to stress. Unicellular yeast cells undergo gross changes in their gene regulation and elongate to form branched filament structures consistin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d1cdf246bd7d7f65dd5645dfc676e0ea
https://pubmed.ncbi.nlm.nih.gov/30271894

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Using Spinach aptamer to correlate mRNA and protein levels in Escherichia coli

Autor: Georgios, Pothoulakis, Tom, Ellis

Publikováno v: Methods in enzymology. 550

In vivo gene expression measurements have traditionally relied on fluorescent proteins such as green fluorescent protein (GFP) with the help of high-sensitivity equipment such as flow cytometers. However, fluorescent proteins report only on the prote

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d9bf34953169b7b33e64b51084f08302
https://pubmed.ncbi.nlm.nih.gov/25605386

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Using Spinach Aptamer to Correlate mRNA and Protein Levels in Escherichia coli

Autor: Georgios Pothoulakis, Tom Ellis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4d9c81bb724f676883cef417ceca0178
https://doi.org/10.1016/bs.mie.2014.10.047

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The spinach RNA aptamer as a characterization tool for synthetic biology

Autor: Francesca Ceroni, Georgios Pothoulakis, Tom Ellis, Benjamin Reeve

Characterization of genetic control elements is essential for the predictable engineering of synthetic biology systems. The current standard for in vivo characterization of control elements is through the use of fluorescent reporter proteins such as

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c653076ca798950b6fa5210eb7a9de77
http://hdl.handle.net/10044/1/31368

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