Zobrazeno 1 - 2
of 2
pro vyhledávání: '"George L. Khaspekov"'
Autor:
Konstantin A. Lukyanov, Eugene D. Sverdlov, Nadya G. Gurskaya, Sergey Lukyanov, Alex Chenchik, Paul D. Siebert, Vagner Ll, Luda Diatchenko, Olga D. Ermolaeva, George L. Khaspekov
Publikováno v:
Analytical Biochemistry. 240:90-97
The major drawback of subtractive cDNA libraries is that the original disproportion in concentrations of different types of transcripts is preserved. This usually makes the isolation of specific rare transcripts extremely difficult. To overcome this
Autor:
Alex S. Shcheglov, Ekaterina A. Bogdanova, Valery B. Kozhemyako, Sergey Lukyanov, George L. Khaspekov, Leonid L. Moroz, Ella A. Meleshkevitch, Mikhail V. Matz, Vagner Ll, Dmitry A. Shagin, Pavel A. Zhulidov
Publikováno v:
Nucleic Acids Research. 32:37e-37
We developed a novel simple cDNA normalization method [termed duplex-specific nuclease (DSN) normalization] that may be effectively used for samples enriched with full-length cDNA sequences. DSN normalization involves the denaturation-reassociation o