Výsledky vyhledávání - "George Huszczynski"

Comparative Evaluation of Genomic and Laboratory Approaches for Determination of Shiga Toxin Subtypes in Escherichia coli

Autor: Amit Mathews, Catherine D. Carrillo, Burton W. Blais, Adam Koziol, Kingsley K. Amoako, Dominic Lambert, Noriko Goji, Martine Gauthier, George Huszczynski

Publikováno v: Journal of Food Protection. 79:2078-2085

The determination of Shiga toxin (ST) subtypes can be an important element in the risk characterization of foodborne ST-producing Escherichia coli (STEC) isolates for making risk management decisions. ST subtyping methods include PCR techniques based

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58ca016315ceee0f530bb3f1e263da2d
https://doi.org/10.4315/0362-028x.jfp-16-228

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Microfluidic Integration of a Cloth-Based Hybridization Array System (CHAS) for Rapid, Colorimetric Detection of Enterohemorrhagic Escherichia coli (EHEC) Using an Articulated, Centrifugal Platform

Autor: Lucas Poncelet, Xuyen Dai Hoa, George Huszczynski, Burton W. Blais, Liviu Clime, Nathalie Corneau, Teodor Veres, Matthias Geissler, Keith Morton, Harold Hebert, Martine Gauthier, Maxence Mounier, Mylène Deschênes

Publikováno v: Analytical Chemistry. 87:10565-10572

We describe the translation of a cloth-based hybridization array system (CHAS), a colorimetric DNA detection method that is used by food inspection laboratories for colony screening of pathogenic agents, onto a microfluidic chip format. We also intro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69b7d03e99ecaa3063cbf5030edc41a5
https://doi.org/10.1021/acs.analchem.5b03085

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Enumeration of Escherichia coli O157:H7 in Outbreak-Associated Beef Patties

Autor: George Huszczynski, Alexander Gill

Publikováno v: Journal of Food Protection. 79:1266-1268

An outbreak of five cases of Escherichia coli O157 infection that occurred in Canada in 2012 was linked to frozen beef patties seasoned with garlic and peppercorn. Unopened retail packs of beef patties from the implicated production lot were recovere

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b6ec642f5aff80f643cf980d1362639
https://doi.org/10.4315/0362-028x.jfp-15-521

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Enterohemorrhagic Escherichia coli Colony Check Assay for the Identification of Serogroups O26, O45, O103, O111, O121, O145, and O157 Colonies Isolated on Plating Media

Autor: Burton W. Blais, Martine Gauthier, Mylène Deschênes, George Huszczynski

Publikováno v: Journal of Food Protection. 77:1212-1218

A simple immunoenzymatic enterohemorrhagic Escherichia coli (EHEC) colony check (ECC) assay was developed for the presumptive identification of priority EHEC colonies isolated on plating media from enrichment broth cultures of foods. With this approa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::782a944c7df7aa3e506d0c6b336c2ae8
https://doi.org/10.4315/0362-028x.jfp-13-555

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Evaluation of eight agar media for the isolation of shiga toxin—Producing Escherichia coli

Autor: Burton W. Blais, George Huszczynski, Alexander Gill, Martine Gauthier

Publikováno v: Journal of Microbiological Methods. 96:6-11

The growth characteristics of 96 shiga toxin-producing Escherichia coli (STEC) strains representing 36 different O-types (including priority O types O26, O45, O103, O111, O121, O145 and O157) on commercial and in-house agar media were studied. The ab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41d21a0d89610156bd98edf323177a04
https://doi.org/10.1016/j.mimet.2013.10.022

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PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain

Autor: Dominic Lambert, George Huszczynski, Martine Gauthier, Michael Knowles, Burton W. Blais

Publikováno v: Journal of food protection. 78(9)

Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6475252d429366f0a0eeac99ffb9fd2
https://pubmed.ncbi.nlm.nih.gov/26319729

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A new pyrosequencing assay for rapid detection and genotyping of Shiga toxin, intimin and O157-specific rfbE genes of Escherichia coli

Autor: Morag Graham, Chad R. Laing, Kingsley K. Amoako, George Huszczynski, Amit Mathews, Noriko Goji, Victor P. J. Gannon

Publikováno v: Journal of microbiological methods. 109

Shiga toxin (stx)-producing Escherichia coli (STEC) contamination in food and water is one of the most recognized concerns and a major financial burden in human hygiene control worldwide. Rapid and highly reliable methods of detecting and identifying

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::796438610d9ba5679c5737606ab72e83
https://pubmed.ncbi.nlm.nih.gov/25523243

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Method for the detection of priority Shiga toxin-producing Escherichia coli in beef trim

Autor: Sam Mohajer, George Huszczynski, Burton W. Blais, Martine Gauthier, Alexander Gill

Publikováno v: Journal of food protection. 76(10)

A method has been developed for the detection in beef trim of priority Shiga toxin-producing E. coli (STEC) strains, defined as E. coli possessing the virulence factors stx1 and/or stx2 and intimin (eae), with O serogroups O26, O45, O103, O111, O121,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7afc3316639c032250005595ec3d120
https://pubmed.ncbi.nlm.nih.gov/24112567

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Polyester cloth-based hybridization array system for identification of enterohemorrhagic Escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157

Autor: Martine Gauthier, Burton W. Blais, Mylène Deschênes, George Huszczynski

Publikováno v: Journal of food protection. 75(9)

A cloth-based hybridization array system (CHAS) was developed for the identification of foodborne colony isolates of seven priority enterohemorrhagic Escherichia coli (EHEC-7) serogroups targeted by U. S. food inspection programs. Gene sequences asso

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c4ac2dba5c7d26a837a2ac1168d09a5
https://pubmed.ncbi.nlm.nih.gov/22947477

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