Zobrazeno 1 - 10
of 11
pro vyhledávání: '"George Crombie"'
Autor:
Gordon L. Snider, Carl Franzblau, Julianne Bryan-Rhadfi, George Crombie, David E. Ciccolella, Edgar C. Lucey, Phillip J. Stone, Barbara Faris
Publikováno v:
American Review of Respiratory Disease. 144:284-290
The accuracy of methods employed to measure the elastin-specific crosslinks, desmosine (DES) and isodesmosine (IDES), has been called into question because contaminants in the urine may cause elevated values. In the present study urine samples were s
Publikováno v:
Biochemistry. 11:3412-3418
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 640:365-367
Egg shell membrane protein contains significant quantities of the lysine-derived aldehyde, allysine, and its aldol condensation product. NaB3H4 reduction followed by alkaline hydrolysis of purified protein revealed that there were six residues/1000 o
Publikováno v:
Analytical biochemistry. 88(2)
A new assay procedure for the determination of collagenolytic activity is presented. The substrate can be prepared by simple reduction of the purified acidsoluble rat tail tendon collagen with NaB3H4. Collagenase activity is determined by measurement
Autor:
Barbara Faris, Robert Shapiro, George Crombie, Carl Franzblau, Paul V. Voynow, Judith Ann Foster
Publikováno v:
Biochemistry. 14(24)
Tropoelastin was isolated from the aortas of chicks rendered lathyritic by treatment with beta-aminopropionitrile. The soluble elastin was judged homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis and possessed an estimated mole
Autor:
Carl Franzblau, Barbara Faris, Michael Glembourtt, Rocco Ferrera, George Crombie, Peter J. Mogayzel
Publikováno v:
Analytical biochemistry. 114(1)
The lysine-derived crosslinks in elastin, desmosine, and isodesmosine, are quantitated in tissue hydrolysates by monitoring high-performance liquid chromatography eluents at 275 nm. The results from this method compare favorably with results from the
Publikováno v:
Analytical biochemistry. 80(2)
A procedure to quantitate trace amounts of elastase in tissue or cell homogenate preparations is described. The procedure is a modification of a method employing NaB3H4-reduced elastin and it does not restrict assay volume. The assay is specific and
Publikováno v:
Journal of biochemical and biophysical methods. 15(5)
This report describes a method for determining specifically and sensitively the degradation of the elastin component within complicated extracellular matrices in vitro. Extracellular matrices rich in elastin were metabolically labeled with [ 3 H]lysi
Publikováno v:
Biochemical and biophysical research communications. 52(4)
Significant amounts of the tetrapeptide Gly-Val-Pro-Gly have been isolated from an elastase-nagarse digest of elastin. A novel method is described for the automated sequencing of the peptide through the attachment of a polar group to the C-terminal r
Publikováno v:
Biochemistry. 10(22)