Zobrazeno 1 - 10
of 152
pro vyhledávání: '"George B Witman"'
Publikováno v:
PLoS ONE, Vol 17, Iss 12, p e0278972 (2022)
Chlamydomonas reinhardtii is an important model organism for the study of many cellular processes, and protein tagging is an increasingly indispensable tool for these studies. To circumvent the disadvantages of conventional approaches in creating a t
Externí odkaz:
https://doaj.org/article/1c79f0762f49404cb6b048f8d915d38f
Autor:
Tyler Picariello, Yuqing Hou, Tomohiro Kubo, Nathan A McNeill, Haru-Aki Yanagisawa, Toshiyuki Oda, George B Witman
Publikováno v:
PLoS ONE, Vol 15, Iss 5, p e0232594 (2020)
Generation and subsequent analysis of mutants is critical to understanding the functions of genes and proteins. Here we describe TIM, an efficient, cost-effective, CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomo
Externí odkaz:
https://doaj.org/article/96a3b69401304c08a21069307eb34f8a
Autor:
Jenna L Wingfield, Ilaria Mengoni, Heather Bomberger, Yu-Yang Jiang, Jonathon D Walsh, Jason M Brown, Tyler Picariello, Deborah A Cochran, Bing Zhu, Junmin Pan, Jonathan Eggenschwiler, Jacek Gaertig, George B Witman, Peter Kner, Karl Lechtreck
Publikováno v:
eLife, Vol 6 (2017)
Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic proteins in cilia. To study how trains assemble, we employed fluorescence protein-tagged IFT proteins in Chlamydomonas reinhardtii. IFT-A and motor
Externí odkaz:
https://doaj.org/article/d5e81191a8b840c585d4a90e0e20d613
Autor:
Jason M Brown, Matthew Mosley, Daniela Montes-Berrueta, Yuqing Hou, Fan Yang, Chasity Scarbrough, George B Witman, Maureen Wirschell
Publikováno v:
PLoS ONE, Vol 12, Iss 3, p e0173842 (2017)
We have used an insertional mutagenesis approach to generate new C. reinhardtii motility mutants. Of 56 mutants isolated, one is a new allele at the ODA3 locus, called oda3-6. Similar to the previously characterized oda3 alleles, oda3-6 has a slow-je
Externí odkaz:
https://doaj.org/article/8029c200a8754a51beafc2bf20f0f239
Autor:
George B. Witman, David R. Mitchell
Publikováno v:
The Chlamydomonas Sourcebook ISBN: 9780128225080
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fff4f437729e0875308142fefcf0f66f
https://doi.org/10.1016/b978-0-12-822508-0.00016-2
https://doi.org/10.1016/b978-0-12-822508-0.00016-2
Autor:
Lea M. Alford, Prachee Avasthi, Mayanka Awasthi, Philip V. Bayly, Robert A. Bloodgood, Alan Brown, Khanh Huy Bui, Dario Cortese, Carol Dieckmann, Dennis Diener, Susan K. Dutcher, Paul Guichard, Virginie Hamel, Peter Hegemann, Masafumi Hirono, Ritsu Kamiya, Stephen M. King, Georg Kreimer, Karl Lechtreck, Paul A. Lefebvre, Esben Lorentzen, Moe R. Mahjoub, David R. Mitchell, Daniela Nicastro, Masayuki Onishi, Junmin Pan, Mary E. Porter, Lynne M. Quarmby, Peeyush Ranjan, Winfield S. Sale, William J. Snell, Ken-ichi Wakabayashi, Kirsty Y. Wan, Maureen Wirschell, George B. Witman, Louis G. Woodhams, Toshiki Yagi, Ryosuke Yamamoto, Pinfen Yang, Yanhe Zhao
Publikováno v:
The Chlamydomonas Sourcebook ISBN: 9780128225080
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::145359ceb52fe8396439fc8ca2abb5ba
https://doi.org/10.1016/b978-0-12-822508-0.00022-8
https://doi.org/10.1016/b978-0-12-822508-0.00022-8
Autor:
Bryony Braschi, Heymut Omran, George B. Witman, Gregory J. Pazour, K. Kevin Pfister, Elspeth A. Bruford, Stephen M. King
Publikováno v:
The Journal of Cell Biology
In this review, Braschi et al. provide an updated consensus nomenclature for components of dynein motors and their assembly factors.
Dyneins are highly complex, multicomponent, microtubule-based molecular motors. These enzymes are responsible fo
Dyneins are highly complex, multicomponent, microtubule-based molecular motors. These enzymes are responsible fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6df41cc6dce7a9e2be24453774a1e7a6
https://www.repository.cam.ac.uk/handle/1810/333886
https://www.repository.cam.ac.uk/handle/1810/333886
Autor:
Daniela Nicastro, Kai Cai, Lei Zhao, George B. Witman, Yuqing Hou, Yanhe Zhao, Nhan Phan, Xi Cheng
Publikováno v:
J Cell Sci
Motile cilia have a ‘9+2’ structure containing nine doublet microtubules and a central apparatus (CA) composed of two singlet microtubules with associated projections. The CA plays crucial roles in regulating ciliary motility. Defects in CA assem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56d27881ef0ad0fab530aa4dfb589806
https://doi.org/10.1242/jcs.254227
https://doi.org/10.1242/jcs.254227
Autor:
Gang Fu, Zhiguo Shang, Erin E. Dymek, Elizabeth F. Smith, Kangkang Song, Daniela Nicastro, Nhan Phan, George B. Witman, Yuqing Hou, Lei Zhao
Publikováno v:
The Journal of Cell Biology
Fu et al. use a WT versus mutant comparison and cryo-electron tomography of Chlamydomonas flagella to identify central apparatus (CA) subunits and visualize their location in the native 3D CA structure. This study provides a better understanding of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::974a0f517d35fcf107fb9435f80c7a79
https://doi.org/10.1083/jcb.201906006
https://doi.org/10.1083/jcb.201906006
Publikováno v:
The Journal of Cell Biology
The central apparatus is an essential component of “9+2” cilia. Zhao et al. identify more than 40 new potential components of the central apparatus of Chlamydomonas. Many are conserved and will facilitate genetic screening of patients with a form
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a1454db262a1c1df9fdf5d6f65b4f13
https://doi.org/10.1083/jcb.201902017
https://doi.org/10.1083/jcb.201902017