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Development of a qualitative real-time RT-PCR assay for the detection of SARS-CoV-2: A guide and case study in setting up an emergency-use, laboratory-developed molecular assay.

Autor: Anahtar MN; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Shaw B; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Slater D; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Byrne E; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Botti-Lodovico Y; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA., Adams G; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Schaffner S; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA., Eversley J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., McGrath G; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Gogakos T; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Lennerz J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Desai Marble H; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Ritterhouse LL; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Batten J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Georgantas NZ; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Pellerin R; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Signorelli S; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Thierauf J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.; Department of Otorhinolaryngology, Head and Neck Surgery, Experimental Head and Neck Oncology, Heidelberg University Hospital, Heidelberg, Germany., Kemball M; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Happi C; Department of Biological Sciences, Redeemer's University, Ede, Osun State, Nigeria.; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria., Grant DS; Viral Hemorrhagic Fever Program, Kenema Government Hospital, Ministry of Health and Sanitation, 1 Combema Road, Kenema, Sierra Leone.; College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown, Sierra Leone., Ndiaye D; African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.; Université Cheikh Anta Diop, BP 5005, Dakar, Sénégal., Siddle KJ; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA., Mehta SB; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, MA, USA., Harris J; Department of Pediatrics, Massachusetts General Hospital for Children, Boston, MA, USA., Ryan ET; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Pierce V; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.; Department of Pediatrics, Massachusetts General Hospital for Children, Boston, MA, USA., LaRocque R; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Lemieux JE; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Sabeti P; Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, USA.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA.; Howard Hughes Medical Institute, Chevy Chase, Maryland.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA., Rosenberg E; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA., Branda J; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts., Turbett SE; Department of Pathology and Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts.; Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.

Publikováno v: MedRxiv : the preprint server for health sciences [medRxiv] 2020 Sep 01. Date of Electronic Publication: 2020 Sep 01.