Výsledky vyhledávání - "Geoffrey D. Parnell"

Structural features of the antibody-A chain linkage that influence the activity and stability of ricin A chain immunotoxins

Autor: Geoffrey D. Parnell, Raymond V. Henry, Alan J. Cumber, Edward J. Wawrzynczak, Brian F. Coles, John H. Westwood

Publikováno v: Bioconjugate Chemistry. 3:397-401

The importance of the various structural elements constituting a ricin A chain immunotoxin to the stability of the disulfide bond between the antibody and A chain was examined using a panel of immunoconjugates prepared with the mouse monoclonal antib

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb6b056c3a8a84bc6996a6ce2569d01a
https://doi.org/10.1021/bc00017a007

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Blood clearance in the rat of a recombinant mouse monoclonal antibody lacking the N-linked oligosaccharide side chains of the CH2 domains

Autor: Alan J. Cumber, Peter T. Jones, Edward J. Wawrzynczak, Geoffrey D. Parnell, Greg Winter

Publikováno v: Molecular Immunology. 29:213-220

The serum half-lives of a wild-type recombinant mouse monoclonal antibody of the IgG2b isotype and a mutant antibody differing from the wild-type antibody by a single amino acid substitution introduced into the CH2 domain, the replacement of Asn 297

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a9d4da70e649139c6e3382328b229fd
https://doi.org/10.1016/0161-5890(92)90102-4

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Recombinant mouse monoclonal antibodies with single amino acid substitutions affecting C1q and high affinity Fc receptor binding have identical serum half-lives in the BALB/c mouse

Autor: Peter T. Jones, Alan J. Cumber, Sylvia Denham, Edward J. Wawrzynczak, Greg Winter, Geoffrey D. Parnell

Publikováno v: Molecular Immunology. 29:221-227

The serum half-lives of three recombinant mouse monoclonal antibodies, differing radically in their ability to bind to C1q or FcRI but only minimally in structure, were determined in the BALB/c mouse following intravenous administration. The wild-typ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a25be0a99f2f68fd6dba402eda5595b5
https://doi.org/10.1016/0161-5890(92)90103-5

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Blocked and non-blocked ricin immunotoxins against the CD4 antigen exhibit higher cytotoxic potency than a ricin A chain immunotoxin potentiated with ricin B chain or with a ricin B chain immunotoxin

Autor: Edward J. Wawrzynczak, Raymond V. Henry, Geoffrey D. Parnell, E. Peter Rieber, Alan J. Cumber, Philip E. Thorpe, Graham J. Watson

Publikováno v: Cancer Immunology Immunotherapy. 32:289-295

An immunotoxin consisting of ricin A chain linked to the monoclonal antibody M-T151, recognising the CD4 antigen, was weakly toxic to the human T-lymphoblastoid cell line CEM in tissue culture. The incorporation of [3H]leucine by CEM cells was inhibi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0d0b1f1170eb0be7a96f3be788230ac
https://doi.org/10.1007/bf01789046

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Biochemical, cytotoxic and pharmacokinetic properties of an immunotoxin composed of a mouse monoclonal antibody Fib75 and the ribosome-inactivating protein alpha-sarcin from Aspergillus giganteus

Autor: Raymond V. Henry, Alan J. Cumber, Elaine J. Derbyshire, Edward J. Wawrzynczak, Norbert Ulbrich, Geoffrey D. Parnell

Publikováno v: European Journal of Biochemistry. 196:203-209

An immunotoxin was synthesized by the attachment of alpha-sarcin, the ribosome-inactivating protein derived from the mould Aspergillus giganteus, to a monoclonal mouse IgG2 antibody Fib75. The alpha-sarcin immunotoxin exerted toxic effects in tissue

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf6af2533599444fe1f4a593c8a4574e
https://doi.org/10.1111/j.1432-1033.1991.tb15805.x

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Comparative biochemical, cytotoxic and pharmacokinetic properties of immunotoxins made with native ricin A chain, ricin A1 chain and recombinant ricin A chain

Autor: Edward J. Wawrzynczak, Alan J. Cumber, Raymond V. Henry, Geoffrey D. Parnell

Publikováno v: International journal of cancer. 47(1)

Immunotoxins were constructed by attaching native ricin A chain, ricin A1 chain and recombinant ricin A chain to the mouse monoclonal IgG2a antibody Fib75 by means of a disulphide linkage using the hetero-bifunctional cross-linker SPDP. The Fib75 imm

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d12961dc47d28edb543a18f7b936a59
https://pubmed.ncbi.nlm.nih.gov/1985869

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Purification of immunotoxins containing the ribosome-inactivating proteins gelonin and momordin using high performance liquid immunoaffinity chromatography compared with blue sepharose CL-6B affinity chromatography

Autor: Raymond V. Henry, Alan J. Cumber, Geoffrey D. Parnell, Edward J. Wawrzynczak

Publikováno v: Journal of immunological methods. 135(1-2)

Comparable amounts of the ribosome-inactivating proteins (RIPs) ricin A chain, gelonin and momordin were allowed to bind to Blue Sepharose CL-6B (immobilised Cibacron Blue F3GA) in phosphate buffer, pH 7.5, and were then eluted quantitatively with bu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::626b1f3587e8a3d8370837d070534836
https://pubmed.ncbi.nlm.nih.gov/2273254

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An ineffective monoclonal antibody-ricin A chain conjugate is converted to a tumouricidal agent in vivo by subsequent systemic administration of ricin B chain

Autor: J Styles, Deirdre P. McIntosh, Helen P. Purvies, Geoffrey D. Parnell, C. J. Dean, Alan J. Cumber, J. Anthony Forrester, Suzanne A. Eccles

Publikováno v: Cancer Immunology Immunotherapy. 24

An immunotoxin comprising a tumour-specific monoclonal antibody (11/160) coupled to ricin A chain, although inactive in in vitro cytotoxicity assays against HSNtc sarcoma target cells, was found to be capable of significant tumouricidal activity in s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::370177a1484bfe1a398483e7f73c28ac
https://doi.org/10.1007/bf00199830

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