Zobrazeno 1 - 10
of 5 298
pro vyhledávání: '"Genome engineering"'
Autor:
Jing Li, Qi Wei, Yiqiu Cheng, Dali Kong, Zhe Kong, Yongping Ke, Xiaofei Dang, Jian-Kang Zhu, Hiroaki Shimada, Daisuke Miki
Publikováno v:
BMC Plant Biology, Vol 24, Iss 1, Pp 1-15 (2024)
Abstract Gene targeting (GT) allows precise manipulation of genome sequences, such as knock-ins and sequence substitutions, but GT in seed plants remains a challenging task. Engineered sequence-specific nucleases (SSNs) are known to facilitate GT via
Externí odkaz:
https://doaj.org/article/8c0974def0714887ac55cd3bc5207f01
Autor:
Ethan Deitcher, Kirk Trisler, Branden S. Moriarity, Caleb J. Bostwick, Fleur A. D. Leenen, Steven R. Deitcher
Publikováno v:
BioMedInformatics, Vol 4, Iss 2, Pp 1384-1395 (2024)
Genome engineered B-cells are being developed for chronic, systemic in vivo protein replacement therapies and for localized, tumor cell-actuated anticancer therapeutics. For continuous systemic engineered protein production, expression may be driven
Externí odkaz:
https://doaj.org/article/767cf045505c4fcfb611e91e3b010a26
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
CRISPR–Cas technologies contribute to enhancing our understanding of plant gene functions, and to the precise breeding of crop traits. Here, we review the latest progress in plant genome editing, focusing on emerging CRISPR–Cas systems, DNA-free
Externí odkaz:
https://doaj.org/article/b9dcae64a1684f6c9be677b06edb5dec
Autor:
Shivani Goolab, Janine Scholefield
Publikováno v:
Frontiers in Genome Editing, Vol 6 (2024)
The designer nuclease, CRISPR-Cas9 system has advanced the field of genome engineering owing to its programmability and ease of use. The application of these molecular scissors for genome engineering earned the developing researchers the Nobel prize
Externí odkaz:
https://doaj.org/article/e4107a19580f44f5ad2ea4a586347f14
Autor:
Patrick J. Lariviere, A. H. M. Zuberi Ashraf, Lucio Navarro-Escalante, Sean P. Leonard, Laurel G. Miller, Nancy A. Moran, Jeffrey E. Barrick
Publikováno v:
mBio, Vol 15, Iss 9 (2024)
ABSTRACT Mechanistic understanding of interactions in many host-microbe systems, including the honey bee microbiome, is limited by a lack of easy-to-use genome engineering approaches. To this end, we demonstrate a one-step genome engineering approach
Externí odkaz:
https://doaj.org/article/e01b36d64072430397920f0252eda892
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-24 (2024)
Abstract Background The improvement of yeast tolerance to acetic, butyric, and octanoic acids is an important step for the implementation of economically and technologically sustainable bioprocesses for the bioconversion of renewable biomass resource
Externí odkaz:
https://doaj.org/article/35a34182e80e4a82971133966a819da1
Autor:
Shankar Subramaniam, Metin Akay, Mark A. Anastasio, Vasudev Bailey, David Boas, Paolo Bonato, Ashutosh Chilkoti, Jennifer R. Cochran, Vicki Colvin, Tejal A. Desai, James S. Duncan, Frederick H. Epstein, Stephanie Fraley, Cecilia Giachelli, K. Jane Grande-Allen, Jordan Green, X. Edward Guo, Isaac B. Hilton, Jay D. Humphrey, Chris R Johnson, George Karniadakis, Michael R. King, Robert F. Kirsch, Sanjay Kumar, Cato T. Laurencin, Song Li, Richard L. Lieber, Nigel Lovell, Prashant Mali, Susan S. Margulies, David F. Meaney, Brenda Ogle, Bernhard Palsson, Nicholas A. Peppas, Eric J. Perreault, Rick Rabbitt, Lori A. Setton, Lonnie D. Shea, Sanjeev G. Shroff, Kirk Shung, Andreas S. Tolias, Marjolein C.H. van der Meulen, Shyni Varghese, Gordana Vunjak-Novakovic, John A. White, Raimond Winslow, Jianyi Zhang, Kun Zhang, Charles Zukoski, Michael I. Miller
Publikováno v:
IEEE Open Journal of Engineering in Medicine and Biology, Vol 5, Pp 1-13 (2024)
Over the past two decades Biomedical Engineering has emerged as a major discipline that bridges societal needs of human health care with the development of novel technologies. Every medical institution is now equipped at varying degrees of sophistica
Externí odkaz:
https://doaj.org/article/99aa19c8944b4368939a87375caa3abd
Publikováno v:
BMC Biology, Vol 22, Iss 1, Pp 1-16 (2024)
Abstract Background Precise gene targeting (GT) is a powerful tool for heritable precision genome engineering, enabling knock-in or replacement of the endogenous sequence via homologous recombination. We recently established a CRISPR/Cas9-mediated ap
Externí odkaz:
https://doaj.org/article/776f13fe3d004247a71e92560a6f23b2
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-11 (2024)
Abstract Background Antibiotic-based plasmid selection and maintenance is a core tool in molecular biology; however, while convenient, this strategy has numerous drawbacks for biological manufacturing. Overuse of antibiotics and antibiotic resistance
Externí odkaz:
https://doaj.org/article/5e5632d23db5472a8140ecf2af420a87