Sequencing by avidity enables high accuracy with low reagent consumption

Autor: Sinan Arslan, Francisco J. Garcia, Minghao Guo, Matthew W. Kellinger, Semyon Kruglyak, Jake A. LeVieux, Adeline H. Mah, Haosen Wang, Junhua Zhao, Chunhong Zhou, Andrew Altomare, John Bailey, Matthew B. Byrne, Chiting Chang, Steve X. Chen, Byungrae Cho, Claudia N. Dennler, Vivian T. Dien, Derek Fuller, Ryan Kelley, Omid Khandan, Michael G. Klein, Michael Kim, Bryan R. Lajoie, Bill Lin, Yu Liu, Tyler Lopez, Peter T. Mains, Andrew D. Price, Samantha R. Robertson, Hermes Taylor-Weiner, Ramreddy Tippana, Austin B. Tomaney, Su Zhang, Minna Abtahi, Mark R. Ambroso, Rosita Bajari, Ava M. Bellizzi, Chris B. Benitez, Daniel R. Berard, Lorenzo Berti, Kelly N. Blease, Angela P. Blum, Andrew M. Boddicker, Leo Bondar, Chris Brown, Chris A. Bui, Juan Calleja-Aguirre, Kevin Cappa, Joshua Chan, Victor W. Chang, Katherine Charov, Xiyi Chen, Rodger M. Constandse, Weston Damron, Mariam Dawood, Nicole DeBuono, John D. Dimalanta, Laure Edoli, Keerthana Elango, Nikka Faustino, Chao Feng, Matthew Ferrari, Keith Frankie, Adam Fries, Anne Galloway, Vlad Gavrila, Gregory J. Gemmen, James Ghadiali, Arash Ghorbani, Logan A. Goddard, Adriana Roginski Guetter, Garren L. Hendricks, Jendrik Hentschel, Daniel J. Honigfort, Yun-Ting Hsieh, Yu-Hsien Hwang Fu, Scott K. Im, Chaoyi Jin, Shradha Kabu, Daniel E. Kincade, Shawn Levy, Yu Li, Vincent K. Liang, William H. Light, Jonathan B. Lipsher, Tsung-li Liu, Grace Long, Rui Ma, John M. Mailloux, Kyle A. Mandla, Anyssa R. Martinez, Max Mass, Daniel T. McKean, Michael Meron, Edmund A. Miller, Celyne S. Moh, Rachel K. Moore, Juan Moreno, Jordan M. Neysmith, Cassandra S. Niman, Jesus M. Nunez, Micah T. Ojeda, Sara Espinosa Ortiz, Jenna Owens, Geoffrey Piland, Daniel J. Proctor, Josua B. Purba, Michael Ray, Daisong Rong, Virginia M. Saade, Sanchari Saha, Gustav Santo Tomas, Nicholas Scheidler, Luqmanal H. Sirajudeen, Samantha Snow, Gudrun Stengel, Ryan Stinson, Michael J. Stone, Keoni J. Sundseth, Eileen Thai, Connor J. Thompson, Marco Tjioe, Christy L. Trejo, Greg Trieger, Diane Ni Truong, Ben Tse, Benjamin Voiles, Henry Vuong, Jennifer C. Wong, Chiung-Ting Wu, Hua Yu, Yingxian Yu, Ming Yu, Xi Zhang, Da Zhao, Genhua Zheng, Molly He, Michael Previte

Publikováno v: Nature Biotechnology.

We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::134ecc28f29d15d85c9ac30057097e39
https://doi.org/10.1038/s41587-023-01750-7

RNA sequencing by direct tagmentation of RNA/DNA hybrids

Autor: Kaiqin Lao, Lin Di, Guanbo Wang, Yanyi Huang, Jiacheng Yao, Raymond W. Lee, X. Sunney Xie, Yue Sun, Lu Liu, Jun Xu, Jie Li, Juntaek Oh, Genhua Zheng, Jianbin Wang, Yalei Wu, Dong Wang, Yusi Fu

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance RNA sequencing is widely used to measure gene expression in biomedical research; therefore, improvements in the simplicity and accuracy of the technology are desirable. All existing RNA sequencing methods rely on the conversion of RNA in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e559f68bb02de877c508a7ad42eaf8e8
http://europepmc.org/articles/PMC7022195

RNA sequencing by direct tagmentation of RNA/DNA hybrids.

Autor: Lin Di, Yusi Fu, Yue Sun, Jie Li, Lu Liu, Jiacheng Yao, Guanbo Wang, Yalei Wu, Kaiqin Lao, Lee, Raymond W., Genhua Zheng, Jun Xu, Juntaek Oh, Dong Wang, Xie, X. Sunney, Yanyi Huang, Jianbin Wang

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America; 2/11/2020, Vol. 117 Issue 6, p2886-2893, 8p

Versatile Synthesis and Rational Design of Caged Morpholinos

Autor: Wen Hong Li, James K. Chen, Genhua Zheng, Surajit Sinha, Andrew Olson, Xiaohu Ouyang, Ilya A. Shestopalov, Cameron L W Pitt

Publikováno v: Journal of the American Chemical Society

Embryogenesis is regulated by genetic programs that are dynamically executed in a stereotypic manner, and deciphering these molecular mechanisms requires the ability to control embryonic gene function with similar spatial and temporal precision. Chem

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66fe0c333be7a2d4fe83c6dc853516a0
https://doi.org/10.1021/ja809933h

One-Pot Synthesis of Micelles with a Cross-Linked Poly(acrylic acid) Core

Autor: Genhua Zheng, Quan Zheng, Cai-Yuan Pan

Publikováno v: Macromolecular Chemistry and Physics. 207:216-223

Stable micelles with polystyrene (PS) as a shell and cross-linked poly[(acrylic acid)-co-(ethylene glycol diacrylate)] as a core have been successfully prepared by reversible addition fragmentation chain transfer (RAFT) copolymerization of acrylic ac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::76f8236d4dc3f8342526d4ba952244fd
https://doi.org/10.1002/macp.200500428