Structure of the replication regulator Sap1 reveals functionally important interfaces

Autor: Maria M. Jørgensen, Babatunde Ekundayo, Mikel Zaratiegui, Karen Skriver, Geneviève Thon, Thomas Schalch

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)

Abstract The mechanism by which specific protein-DNA complexes induce programmed replication fork stalling in the eukaryotic genome remains poorly understood. In order to shed light on this process we carried out structural investigations on the esse

Externí odkaz: https://doaj.org/article/928c8bb1f12547a5aec8f2641195749b

Nucleation and spreading of a heterochromatic domain in fission yeast

Autor: Michaela J. Obersriebnig, Emil M. H. Pallesen, Kim Sneppen, Ala Trusina, Geneviève Thon

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016)

Chromosomes contain large heterochromatin domains. Here, the authors measure the kinetics of heterochromatin formation in fission yeast and show both global and local feedbacks by nucleosome-bound enzymes are important for formation and stability of

Externí odkaz: https://doaj.org/article/8880659a3dfa477c9b75c47c49059d14

Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange

Autor: Takahisa Maki, Geneviève Thon, Hiroshi Iwasaki

A haploid of the fission yeastSchizosaccharomyces pombeexpresses either the P or M matingtype, determined by the active, euchromatic,mat1cassette. Mating-type is switched by Rad51-driven gene conversion ofmat1using a heterochromatic donor cassette,ma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5fb7f3deb428a847bdf08dffd06abbce
https://doi.org/10.1101/2022.11.22.517464

Establishment of heterochromatin in domain-size-dependent bursts

Autor: Sif Christine Lykke Hougaard, Geneviève Thon, Jan Fabio Nickels, Ala Trusina, Amanda Møller Mortensen, Kim Sneppen, Sebastian Jespersen Charlton, Ashleigh Katrine Edwards

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences
Nickels, J F, Edwards, A K, Charlton, S J, Mortensen, A M, Hougaard, S C L, Trusina, A, Sneppen, K & Thon, G 2021, ' Establishment of heterochromatin in domain-size-dependent bursts ', Proceedings of the National Academy of Sciences of the United States of America, vol. 118, no. 15, e2022887118 . https://doi.org/10.1073/pnas.2022887118

Significance How repressive heterochromatic states propagate along chromosomes and are subsequently maintained for many cell divisions remain important unanswered questions in biology. Combining mathematical modeling and single-cell measurements, we

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dad7e03626182a75a67a0330564532a2
https://doi.org/10.1073/pnas.2022887118

Two portable recombination enhancers direct donor choice in fission yeast heterochromatin.

Autor: Tadas Jakočiūnas, Lærke Rebekka Holm, Janne Verhein-Hansen, Ala Trusina, Geneviève Thon

Publikováno v: PLoS Genetics, Vol 9, Iss 10, p e1003762 (2013)

Mating-type switching in fission yeast results from gene conversions of the active mat1 locus by heterochromatic donors. mat1 is preferentially converted by mat2-P in M cells and by mat3-M in P cells. Here, we report that donor choice is governed by

Externí odkaz: https://doaj.org/article/e509af0c255044b09a44722e83531786