A truncated anti-CRISPR protein prevents spacer acquisition but not interference

Autor: Cécile Philippe, Carlee Morency, Pier-Luc Plante, Edwige Zufferey, Rodrigo Achigar, Denise M. Tremblay, Geneviève M. Rousseau, Adeline Goulet, Sylvain Moineau

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)

Phages can use ACR proteins that inhibit the adaptive immunity activities of bacterial CRISPR-Cas systems. Here, Philippe et al. show that these systems can block ACR-containing phages by targeting the acr gene, and this can select for phage mutants

Externí odkaz: https://doaj.org/article/ead3f2e462bc45f681529084f0c6c6d4

Author Correction: A truncated anti-CRISPR protein prevents spacer acquisition but not interference

Autor: Cécile Philippe, Carlee Morency, Pier-Luc Plante, Edwige Zufferey, Rodrigo Achigar, Denise M. Tremblay, Geneviève M. Rousseau, Adeline Goulet, Sylvain Moineau

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-2 (2022)

Externí odkaz: https://doaj.org/article/1cbf6bb71fd9461d8834ae23403e9e70

Ectopic Spacer Acquisition in Streptococcus thermophilus CRISPR3 Array

Autor: Rodrigo Achigar, Martina Scarrone, Geneviève M. Rousseau, Cécile Philippe, Felipe Machado, Valentina Duvós, María Pía Campot, Moïra B. Dion, Yuyu Shao, María Julia Pianzzola, Sylvain Moineau

Publikováno v: Microorganisms, Vol 9, Iss 3, p 512 (2021)

Streptococcus thermophilus relies heavily on two type II-A CRISPR-Cas systems, CRISPR1 and CRISPR3, to resist siphophage infections. One hallmark of these systems is the integration of a new spacer at the 5′ end of the CRISPR arrays following phage

Externí odkaz: https://doaj.org/article/41ad0a89482b4df0b6aaff6215969e36

Characterization of the Escherichia coli Virulent Myophage ST32

Autor: Honghui Liu, Hany Geagea, Geneviève M. Rousseau, Simon J. Labrie, Denise M. Tremblay, Xinchun Liu, Sylvain Moineau

Publikováno v: Viruses, Vol 10, Iss 11, p 616 (2018)

The virulent phage ST32 that infects the Escherichia coli strain ST130 was isolated from a wastewater sample in China and analyzed. Morphological observations showed that phage ST32 belongs to the Myoviridae family, as it has an icosahedral capsid an

Externí odkaz: https://doaj.org/article/650d397842494a4fa791c4f8bc31be09

CRISPR-Cas provides limited phage immunity to a prevalent gut bacterium in gnotobiotic mice

Autor: Torben Sølbeck Rasmussen, Anna Kirstine Koefoed, Ling Deng, Musemma K. Muhammed, Geneviève M. Rousseau, Witold Kot, Sabrina Sprotte, Horst Neve, Charles M.A.P. Franz, Axel Kornerup Hansen, Finn Kvist Vogensen, Sylvain Moineau, Dennis Sandris Nielsen

Publikováno v: Rasmussen, T S, Koefoed, A K, Deng, L, Muhammed, M K, Rousseau, G M, Kot, W, Sprotte, S, Neve, H, Franz, C M A P, Hansen, A K, Vogensen, F K, Moineau, S & Nielsen, D S 2023, ' CRISPR-Cas provides limited phage immunity to a prevalent gut bacterium in gnotobiotic mice ', ISME Journal, vol. 17, pp. 432–442 . https://doi.org/10.1038/s41396-023-01358-4

Many prokaryotes harbor the adaptive CRISPR-Cas system, which stores small nucleotide fragments from previous invasions of nucleic acids via viruses or plasmids. This molecular archive blocks further invaders carrying identical or similar nucleotide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::936a5e4f180242def03930f516330093
https://curis.ku.dk/portal/da/publications/crisprcas-provides-limited-phage-immunity-to-a-prevalent-gut-bacterium-in-gnotobiotic-mice(44314f1b-810e-4360-80ea-c46333aef11c).html