Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Gene Editing/methods"'
Autor:
Idoko-Akoh, Alewo, McGrew, Michael J
Publikováno v:
Methods in Molecular Biology ISBN: 9781071629895
Idoko-Akoh, A & McGrew, M J 2023, Generation of Genome-Edited Chicken Through Targeting of Primordial Germ Cells . in Methods in Molecular Biology . vol. 2631, 2, Methods in molecular biology (Clifton, N.J.), pp. 419-441 . https://doi.org/10.1007/978-1-0716-2990-1_20
Idoko-Akoh, A & McGrew, M J 2023, Generation of Genome-Edited Chicken Through Targeting of Primordial Germ Cells . in Methods in Molecular Biology . vol. 2631, 2, Methods in molecular biology (Clifton, N.J.), pp. 419-441 . https://doi.org/10.1007/978-1-0716-2990-1_20
Genome editing technology facilitates the creation of specific and precise genetic changes to unravel gene function and rapidly transfer unique alleles between chicken breeds in contrast to lengthy traditional crossbreeding methods for the study of p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ea3eba699d26226f9fa372a018ad8d3
https://doi.org/10.1007/978-1-0716-2990-1_20
https://doi.org/10.1007/978-1-0716-2990-1_20
Autor:
Jason Micklefield, Ying Zhuo, Katherine J Robins, Yingxin Zhang, Joanna K Fyans, Brian J. C. Law, Wei Li Thong, Abigail J Herbert
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Thong, W L, Zhang, Y, Zhuo, Y, Robins, K J, Fyans, J K, Herbert, A J, Law, B J C & Micklefield, J 2021, ' Gene editing enables rapid engineering of complex antibiotic assembly lines ', Nature Communications, vol. 12, no. 1, 6872 . https://doi.org/10.1038/s41467-021-27139-1
Nature Communications
Thong, W L, Zhang, Y, Zhuo, Y, Robins, K J, Fyans, J K, Herbert, A J, Law, B J C & Micklefield, J 2021, ' Gene editing enables rapid engineering of complex antibiotic assembly lines ', Nature Communications, vol. 12, no. 1, 6872 . https://doi.org/10.1038/s41467-021-27139-1
Nature Communications
Re-engineering biosynthetic assembly lines, including nonribosomal peptide synthetases (NRPS) and related megasynthase enzymes, is a powerful route to new antibiotics and other bioactive natural products that are too complex for chemical synthesis. H
Publikováno v:
Zhai, R, Zheng, C, Yang, Z, Li, T, Chen, J & Shen, X 2022, ' Contribution of CRISPRable DNA to human complex traits ', Communications biology, vol. 5, no. 1, pp. 1111 . https://doi.org/10.1038/s42003-022-03969-7
CRISPR-Cas is a powerful genome editing tool for various species and human cell lines, widely used in many research areas including studying the mechanisms, targets, and gene therapies of human diseases. Recent developments have even allowed high-thr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bd622b9b51187c9293921f674a3943d
https://hdl.handle.net/20.500.11820/3c33b491-608a-4494-815d-05edf6a788b2
https://hdl.handle.net/20.500.11820/3c33b491-608a-4494-815d-05edf6a788b2
Publikováno v:
STAR protocols, 3(2):101358
CRISPR/Cas9 technology allows accurate, marker-less genome editing. We report a detailed, robust, and streamlined protocol for CRISPR/Cas9 genome editing in Saccharomyces cerevisiae, based on the widely used MoClo-Yeast Toolkit (https://www.addgene.o
Autor:
Harry Begthel, Johan H. van Es, Hans Clevers, Oded Kopper, Celien P.H. Vreuls, Kadi Lõhmussaar, Jeroen Korving
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Nature Communications
Nature Communications, 11(1). Nature Publishing Group
Nature Communications
Nature Communications, 11(1). Nature Publishing Group
High-grade serous ovarian cancer (HG-SOC)—often referred to as a “silent killer”—is the most lethal gynecological malignancy. The fallopian tube (murine oviduct) and ovarian surface epithelium (OSE) are considered the main candidate tissues o
Publikováno v:
Nature Biotechnology, 39(8), 920-921. Nature Publishing Group
Autor:
de la Fuente, Josu, Gluckman, Eliane, Makani, Julie, Telfer, Paul, Faulkner, Lawrence, Corbacioglu, Selim, Paediatric Diseases Working Party of the European Society for Blood and Marrow Transplantation
Publikováno v:
The lancet. Haematology, Vol. 7, No 12 (2020) pp. e902-e911
Sickle cell disease is one of the most common, life-threatening, non-communicable diseases in the world and a major public health problem. Following the implementation of simple preventive and therapeutic modalities, infant mortality has almost been
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=od______1400::2560f167c6ade3eedf78b38156db10e4
https://archive-ouverte.unige.ch/unige:156647
https://archive-ouverte.unige.ch/unige:156647
Publikováno v:
STAR Protocols
STAR Protocols, Vol 1, Iss 3, Pp 100208-(2020)
STAR Protocols, Vol 1, Iss 3, Pp 100208-(2020)
Summary Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49184fd730cbbb7e73f1e52d9e18e2e4
https://hdl.handle.net/10216/145275
https://hdl.handle.net/10216/145275
Autor:
S. Shareef, Victoria Harding, C B T Moore, Kathleen A. Christie, Kevin Blighe, Thomais Kakouli-Duarte, Leandro Castellano, Justin Stebbing, Bo L. Chawes, Rachel S. Kelly, M A Nesbit, Connie Chao-Shern, Jessica Lasky-Su, Larry DeDionisio
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-20 (2018)
Blighe, K, DeDionisio, L, Christie, K A, Chawes, B, Shareef, S, Kakouli-Duarte, T, Chao-Shern, C, Harding, V, Kelly, R S, Castellano, L, Stebbing, J, Lasky-Su, J A, Nesbit, M A & Moore, C B T 2018, ' Gene editing in the context of an increasingly complex genome ', BMC Genomics, vol. 19, no. 1, pp. 595 . https://doi.org/10.1186/s12864-018-4963-8
BMC Genomics
Blighe, K, DeDionisio, L, Christie, K A, Chawes, B, Shareef, S, Kakouli-Duarte, T, Chao-Shern, C, Harding, V, Kelly, R S, Castellano, L, Stebbing, J, Lasky-Su, J A, Nesbit, M A & Moore, C B T 2018, ' Gene editing in the context of an increasingly complex genome ', BMC Genomics, vol. 19, no. 1, pp. 595 . https://doi.org/10.1186/s12864-018-4963-8
BMC Genomics
The reporting of the first draft of the human genome in 2000 brought with it much hope for the future in what was felt as a paradigm shift toward improved health outcomes. Indeed, we have now mapped the majority of variation across human populations
Autor:
Heather A. Hartman, Nicholas J. Ahn, Deepthi Alapati, Kiran Musunuru, Jeremy Katzen, Avery C. Rossidis, Hiaying Li, Michael F. Beers, Edward E. Morrisey, Yaniv Tomer, William J. Zacharias, William H. Peranteau, Su Zhou, Barbara E. Coons, Kshitiz Singh, John D. Stratigis, Alexandra C. Chadwick
Monogenic lung diseases that are caused by mutations in surfactant genes of the pulmonary epithelium are marked by perinatal lethal respiratory failure or chronic diffuse parenchymal lung disease with few therapeutic options. Using a CRISPR fluoresce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f962fe89ad5ef2b700d153b5cdf19ac9
https://europepmc.org/articles/PMC6822403/
https://europepmc.org/articles/PMC6822403/