Inducible Genetic Code Expansion in Eukaryotes

Autor: Christine Koehler, Christopher D. Reinkemeier, Gemma Estrada Girona, Edward A. Lemke

Publikováno v: ChemBioChem
Chembiochem

Genetic code expansion (GCE) is a versatile tool to site‐specifically incorporate a noncanonical amino acid (ncAA) into a protein, for example, to perform fluorescent labeling inside living cells. To this end, an orthogonal aminoacyl‐tRNA‐synth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::349eaa90625e3d2fa51f03ba176819b2
https://doi.org/10.1002/cbic.202000338

Verbesserte Erweiterung des eukaryotischen genetischen Codes für seitenspezifische, hochauflösende Click-PAINT-Mikroskopie

Autor: Camilla Ventura Santos, Ivana Nikić, Christine Koehler, Gemma Estrada Girona, Nataliia V. Shymanska, Jun Hee Kang, Giulia Paci, Sofya Mikhaleva, Daniel Spitz, Edward A. Lemke

Publikováno v: Angewandte Chemie. 128:16406-16410

Hochauflosende Mikroskopie (SRM) profitiert von der Moglichkeit der Einfuhrung kleiner photostabiler Fluoreszenzmarkierungen in Proteine. Die Erweiterung des genetischen Codes (GCE) erfullt diese Anforderung, indem sie es ermoglicht, kleine Markierun

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fbbc65207363199b2d6a7ef99cf37b5d
https://doi.org/10.1002/ange.201608284

Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins

Autor: Eszter, Kozma, Giulia, Paci, Gemma, Estrada Girona, Edward A, Lemke, Péter, Kele

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1728

Tetrazine-bearing fluorescent labels enable site-specific tagging of proteins that are genetically manipulated with dienophile modified noncanonical amino acids. The inverse electron demand Diels-Alder reaction between the tetrazine and the dienophil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2be042704574997466081bf8ff39badf
https://pubmed.ncbi.nlm.nih.gov/29405009

Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements

Autor: Christine Koehler, Edward A. Lemke, Kiersten M. Ruff, Ankur Mishra, Niccolò Banterle, Michael Kachala, Gustavo Fuertes, Santiago Esteban-Martín, Sigrid Milles, Aritra Chowdhury, Davide Mercadante, Frauke Gräter, Patrick Onck, Gemma Estrada Girona, Rohit V. Pappu, Dmitri I. Svergun

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 114(31), E6342-E6351. NATL ACAD SCIENCES

Unfolded states of proteins and native states of intrinsically disordered proteins (IDPs) populate heterogeneous conformational ensembles in solution. The average sizes of these heterogeneous systems, quantified by the radius of gyration (RG ), can b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e07048828a21dfa8c5ec956dad7f9d1e
https://pubmed.ncbi.nlm.nih.gov/28716919

Genetic code expansion for multiprotein complex engineering

Autor: Juri Rappsilber, Martin Jechlinger, Zhuo Angel Chen, Hueseyin Besir, Attila Gyenesei, Mirella Wawryszyn, Kapil Gupta, Ksenija Radic, Juan Zou, Markus Hsi-Yang Fritz, Imre Berger, Jonathan J M Landry, Christine Koehler, Stefan Braese, Vladimir Benes, Paul F. Sauter, Peggy Stolt-Bergner, Piau Siong Tan, Jan O. Korbel, Sini Junttila, Edward A. Lemke, Gemma Estrada Girona, Bence Galik, Carsten Schultz, Jan Erik Hoffmann, Moritz Bosse Biskup, Giancarlo Pruneri

Publikováno v: Koehler, C, Sauter, P F, Wawryszyn, M, Girona, G E, Gupta, K, Landry, J J M, Fritz, M H Y, Radic, K, Hoffmann, J E, Chen, Z A, Zou, J, Tan, P S, Galik, B, Junttila, S, Stolt-Bergner, P, Pruneri, G, Gyenesei, A, Schultz, C, Biskup, M B, Besir, H, Benes, V, Rappsilber, J, Jechlinger, M, Korbel, J O, Berger, I, Braese, S & Lemke, E A 2016, ' Genetic code expansion for multiprotein complex engineering ', Nature Methods, vol. 13, no. 12, pp. 997-1000 . https://doi.org/10.1038/nmeth.4032
Koehler, C, Sauter, P F, Wawryszyn, M, Girona, G E, Gupta, K, Landry, J J M, Fritz, M H Y, Radic, K, Hoffmann, J E, Chen, Z A, Zou, J, Tan, P S, Galik, B, Junttila, S, Stolt-Bergner, P, Pruneri, G, Gyenesei, A, Schultz, C, Biskup, M B, Besir, H, Benes, V, Rappsilber, J, Jechlinger, M, Korbel, J O, Berger, I, Braese, S & Lemke, E A 2016, ' Genetic code expansion for multiprotein complex engineering ', Nature Methods, vol. 13, no. 12, pp. 997–1000 . https://doi.org/10.1038/nmeth.4032

We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c2ba09e5ec8b2f696169ad003dea91a
https://hdl.handle.net/20.500.11820/d43a4262-d2fb-415b-82ab-e8dfb28a0add