N-Methylation of Amino Acids in Gelatinase Biosynthesis-Activating Pheromone Identifies Key Site for Stability Enhancement with Retention of the Enterococcus faecalis fsr Quorum Sensing Circuit Response

Autor: Yftah Tal-Gan, Dominic N McBrayer, Brooke K Gantman

Publikováno v: ACS Infect Dis

The growing prevalence of multiantibiotic-resistant bacteria necessitates looking at potential alternative approaches for attenuating infections by bacteria while reducing the rate of antibiotic resistance development. Enterococcus faecalis is respon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2144502399240e11692b604373ae8340
https://doi.org/10.1021/acsinfecdis.9b00097

An Entirely Solid Phase Peptide Synthesis-Based Strategy for Synthesis of Gelatinase Biosynthesis-Activating Pheromone (GBAP) Analogue Libraries: Investigating the Structure-Activity Relationships of the Enterococcus faecalis Quorum Sensing Signal

Autor: Yftah Tal-Gan, Crissey D Cameron, Brooke K Gantman, Dominic N McBrayer

Publikováno v: Organic letters. 19(12)

The development of an entirely solid-phase peptide synthesis (SPPS)-based synthesis of the quorum sensing signal gelatinase biosynthesis-activating pheromone (GBAP) from Enterococcus faecalis is reported. The method was used to prepare three librarie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5776a5f2269a2e0986a58dfca915911f
https://pubmed.ncbi.nlm.nih.gov/28590764

Structure-Activity Relationship of Gelatinase Biosynthesis-Activating Pheromone of Enterococcus faecalis

Autor: Masaru Tanokura, Koji Nagata, Kenji Sonomoto, Jiro Nakayama, Kenzo Nishiguchi

Publikováno v: Journal of Bacteriology. 191:641-650

The expression of pathogenicity-related extracellular proteases, namely, gelatinase and serine protease, in Enterococcus faecalis is positively regulated by a quorum-sensing system mediated by an autoinducing peptide called gelatinase biosynthesis-ac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e818b559e86dac6c0e9801450bcc5c34
https://doi.org/10.1128/jb.01029-08

Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy

Autor: Simon G. Patching, Mary K. Phillips-Jones, Rohanah Hussain, Giuliano Siligardi

Publikováno v: Biochimica et Biophysica Acta:Biomembranes
Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2014, 1838 (1), pp.34-42. ⟨10.1016/j.bbamem.2013.06.019⟩

A great number of membrane proteins have proven difficult to crystallise for use in X-ray crystallographic structural determination or too complex for NMR structural studies. Circular dichroism (CD) is a fast and relatively easy spectroscopic techniq

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cf80797dd4d9e11942f1cc019a23f2e
https://pubmed.ncbi.nlm.nih.gov/23811229

Anti-HIV siamycin I directly inhibits autophosphorylation activity of the bacterial FsrC quorum sensor and other ATP-dependent enzyme activities

Autor: Kenzo Nishiguchi, Hayley M. Yuille, Pikyee Ma, Lianne M. Davis, Jiro Nakayama, Mary K. Phillips-Jones

Publikováno v: FEBS letters. 585(17)

Siamycin I disrupts growth and quorum sensing in Enterococcus faecalis. Using purified intact protein, we demonstrate here that quorum membrane sensor kinase FsrC is a direct target of siamycin I, reducing pheromone-stimulated autophosphorylation act

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34cb28e82ad7d605fab85aa61624dc98
https://pubmed.ncbi.nlm.nih.gov/21803040