Zobrazeno 1 - 10
of 115
pro vyhledávání: '"Geeta J. Narlikar"'
Autor:
Un Seng Chio, Eugene Palovcak, Anton A. A. Smith, Henriette Autzen, Elise N. Muñoz, Zanlin Yu, Feng Wang, David A. Agard, Jean-Paul Armache, Geeta J. Narlikar, Yifan Cheng
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-12 (2024)
Abstract Single-particle cryo-EM is widely used to determine enzyme-nucleosome complex structures. However, cryo-EM sample preparation remains challenging and inconsistent due to complex denaturation at the air-water interface (AWI). Here, to address
Externí odkaz:
https://doaj.org/article/27aa364ca261463cb826925799de5972
Autor:
Clara Lopes Novo, Emily V. Wong, Colin Hockings, Chetan Poudel, Eleanor Sheekey, Meike Wiese, Hanneke Okkenhaug, Simon J. Boulton, Srinjan Basu, Simon Walker, Gabriele S. Kaminski Schierle, Geeta J. Narlikar, Peter J. Rugg-Gunn
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
Here the authors show satellite transcripts in mouse embryonic stem cells drive HP1α into droplets in vitro and also control HP1α organisation and association with chromatin in vivo. Depleting the satellite transcripts converts heterochromatin into
Externí odkaz:
https://doaj.org/article/9694c15ea6ff49a7afe36d07b4947267
Autor:
Coral Y. Zhou, Caitlin I. Stoddard, Jonathan B. Johnston, Michael J. Trnka, Ignacia Echeverria, Eugene Palovcak, Andrej Sali, Alma L. Burlingame, Yifan Cheng, Geeta J. Narlikar
Publikováno v:
Cell Reports, Vol 19, Iss 10, Pp 2033-2044 (2017)
The hexameric AAA+ ATPases Rvb1 and Rvb2 (Rvbs) are essential for diverse processes ranging from metabolic signaling to chromatin remodeling, but their functions are unknown. While originally thought to act as helicases, recent proposals suggest that
Externí odkaz:
https://doaj.org/article/163ed11f9a7840cd8bc6609903d40b17
Autor:
Madeline M Keenen, David Brown, Lucy D Brennan, Roman Renger, Harrison Khoo, Christopher R Carlson, Bo Huang, Stephan W Grill, Geeta J Narlikar, Sy Redding
Publikováno v:
eLife, Vol 10 (2021)
In mammals, HP1-mediated heterochromatin forms positionally and mechanically stable genomic domains even though the component HP1 paralogs, HP1α, HP1β, and HP1γ, display rapid on-off dynamics. Here, we investigate whether phase-separation by HP1 p
Externí odkaz:
https://doaj.org/article/1e4bc5d189424ef491218ff0e4ff27e9
Autor:
Nour J Abdulhay, Colin P McNally, Laura J Hsieh, Sivakanthan Kasinathan, Aidan Keith, Laurel S Estes, Mehran Karimzadeh, Jason G Underwood, Hani Goodarzi, Geeta J Narlikar, Vijay Ramani
Publikováno v:
eLife, Vol 9 (2020)
Our understanding of the beads-on-a-string arrangement of nucleosomes has been built largely on high-resolution sequence-agnostic imaging methods and sequence-resolved bulk biochemical techniques. To bridge the divide between these approaches, we pre
Externí odkaz:
https://doaj.org/article/3694ff2de5284ae9a0d6610c16155ba7
Autor:
Jean Paul Armache, Nathan Gamarra, Stephanie L Johnson, John D Leonard, Shenping Wu, Geeta J Narlikar, Yifan Cheng
Publikováno v:
eLife, Vol 8 (2019)
The SNF2h remodeler slides nucleosomes most efficiently as a dimer, yet how the two protomers avoid a tug-of-war is unclear. Furthermore, SNF2h couples histone octamer deformation to nucleosome sliding, but the underlying structural basis remains unk
Externí odkaz:
https://doaj.org/article/af8aead7e47340e6a20674406782f5e2
Publikováno v:
eLife, Vol 8 (2019)
In eukaryotes, a first step towards the nuclear DNA compaction process is the formation of a nucleosome, which is comprised of negatively charged DNA wrapped around a positively charged histone protein octamer. Often, it is assumed that the complexat
Externí odkaz:
https://doaj.org/article/086ab7e1068d4d36b8d2f53165901bf5
Autor:
Bryan A. Gibson, Claudia Blaukopf, Tracy Lou, Lifeng Chen, Lynda K. Doolittle, Ilya Finkelstein, Geeta J. Narlikar, Daniel W. Gerlich, Michael K. Rosen
Publikováno v:
Proceedings of the National Academy of Sciences. 120
Nuclear DNA in eukaryotes is wrapped around histone proteins to form nucleosomes on a chromatin fiber. Dynamic folding of the chromatin fiber into loops and variations in the degree of chromatin compaction regulate essential processes such as transcr
Publikováno v:
eLife, Vol 7 (2018)
ISWI family chromatin remodeling motors use sophisticated autoinhibition mechanisms to control nucleosome sliding. Yet how the different autoinhibitory domains are regulated is not well understood. Here we show that an acidic patch formed by histones
Externí odkaz:
https://doaj.org/article/541b8cfc76be4658bd1e9d44fdef24a5
Autor:
Geeta J. Narlikar, Nathan Gamarra
Publikováno v:
Nature Structural & Molecular Biology. 28:548-551