Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Gebhard von Jagow"'
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization tech
Autor:
Gebhard von Jagow, Thomas A. Link
Publikováno v:
Journal of Biological Chemistry. 270:25001-25006
Bovine heart bc1 complex is reversibly inhibited by zinc ions with an inhibition constant KI of 10(-7) M at pHor = 7.0. Binding of zinc is at least a factor of 10 tighter than binding of any other metal ion tested. Essentially complete inhibition of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3966fab8cc6f9db04fcaab05416430b7
https://doi.org/10.1074/jbc.270.42.25001
https://doi.org/10.1074/jbc.270.42.25001
Publikováno v:
European Journal of Biochemistry. 230:235-241
Perinatal development of cytochrome-c oxidase (complex IV) and ubiquinol–cytochrome-c reductase (complex III) was investigated in rat heart and liver by analysing catalytic properties, protein amounts, and subunit isoforms during the transition fro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b09c9b1ebdc332e4a743c6b3f7622a3f
https://doi.org/10.1111/j.1432-1033.1995.0235i.x
https://doi.org/10.1111/j.1432-1033.1995.0235i.x
Autor:
Gebhard von Jagow, Thomas A. Link, Ulrich Brandt, B. M. Geier, Wilfred R. Hagen, Hermann Schägger, Claus Ortwein
Publikováno v:
European Journal of Biochemistry. 227:296-302
A novel, large-scale method for the purification of cytochrome-c oxidase from the yeast Saccharomyces cerevisiae is described. The isolation procedure gave highly pure and active enzyme at high yields. The purified enzyme exhibited a heme a/protein r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9acfe93fc7254f1b418d06a7e2962d7c
https://doi.org/10.1111/j.1432-1033.1995.tb20388.x
https://doi.org/10.1111/j.1432-1033.1995.tb20388.x
Publikováno v:
European Journal of Biochemistry. 208:375-380
Cytochrome-c reductase was isolated from Saccharomyces cerevisiae GM50-3C. A tenth subunit was detected with molecular mass 8.5 kDa on SDS/PAGE. Two yeast mutants selected for resistance to myxothiazol, an inhibitor of the Q(0) center (Q, ubiquinone)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fe430dcd9e14aaea3555b59f35f949c
https://doi.org/10.1111/j.1432-1033.1992.tb17197.x
https://doi.org/10.1111/j.1432-1033.1992.tb17197.x
Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form
Autor:
Gebhard von Jagow, Hermann Schägger
Publikováno v:
Analytical Biochemistry. 199:223-231
A discontinuous electrophoretic system for the isolation of membrane proteins from acrylamide gels has been developed using equipment for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Coomassie dyes were introduced to induce a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fe4ecd6c7c2823e2a965c8d7b68ae8f
https://doi.org/10.1016/0003-2697(91)90094-a
https://doi.org/10.1016/0003-2697(91)90094-a
Autor:
Gebhard von Jagow, Ulrich Brandt
Publikováno v:
FEBS Letters. 287:215-218
Cytochrome c reductase is inhibited by p-chlorophenyl-methoxybenzyl-ketoxime (CPMB-oxime). CPMB-oxime induces a red-shift of the reduced spectrum of cytochrome b. The inhibitor blocks the oxidation of ubihydroquinone at the Qp center of this enzyme i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00cfbe2d0141fbbbb0868dda0e8844f5
https://doi.org/10.1016/0014-5793(91)80054-7
https://doi.org/10.1016/0014-5793(91)80054-7
Autor:
Gebhard von Jagow, Ulrich Brandt
Publikováno v:
European Journal of Biochemistry. 195:163-170
The binding characteristics of inhibitors of the mitochondrial cytochrome c reductase were studied by fluorescence quench titration. Based on the standard binding equation, the applied numerical method allowed the online recorded titration curves to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a09bb3be486daee9ec0ab2031bf90e63
https://doi.org/10.1111/j.1432-1033.1991.tb15690.x
https://doi.org/10.1111/j.1432-1033.1991.tb15690.x
Autor:
Carola Hunte, Gebhard Von Jagow, Aimo Kannt, Reiner Kiefersauer, Werner Kühlbrandt, C. Roy, D. Lancaster, Ehud M Landau, Thomas A. Link, Hartmut Michel, Etana Padan, Hildur Palsdottir, Helmut Reilander, Christoph Reinhart, Hermann Schagger, Tewfik Soulimane, Manuel E. Than, Georgios Tsiotis, Irene Tsirogianni, Miro Venturi, H. Markus Weiss
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c4783ae2bbc0646c45fd535703a9f3f3
https://doi.org/10.1016/b978-012361776-7/50000-0
https://doi.org/10.1016/b978-012361776-7/50000-0
Publikováno v:
Membrane Protein Purification and Crystallization (Second Edition)
Membrane proteins tend to form aggregates even in the presence of detergents, which reduces the efficacy of all separation techniques. It is therefore essential to select an effective solubilizing, but non-denaturing detergent to ascertain the minimu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fcf7bc341e4422fdba3732d369fbbd3
https://doi.org/10.1016/b978-012361776-7/50002-4
https://doi.org/10.1016/b978-012361776-7/50002-4