Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Gaylen A Uhlich"'
Publikováno v:
BioTechniques, Vol 72, Iss 6, Pp 255-262 (2022)
Campylobacter genetics research is negatively impacted by a shortage of molecular tools for expressing DNA elements. A previous technique coupled an antibiotic resistance gene and its promoter to a gene of interest, inserting this expression unit int
Externí odkaz:
https://doaj.org/article/13adef224ff843ebaf01679e6be509da
Autor:
Gaylen A Uhlich, Elisa Andreozzi, Bryan J Cottrell, Erin R Reichenberger, Xinmin Zhang, George C Paoli
Publikováno v:
PLoS ONE, Vol 13, Iss 5, p e0196271 (2018)
The high frequency of prophage insertions in the mlrA gene of clinical serotype O157:H7 isolates renders such strains deficient in csgD-dependent biofilm formation but prophage induction may restore certain mlrA properties. In this study we used tran
Externí odkaz:
https://doaj.org/article/c623de11110746db94686ec7983e61a0
Autor:
Chin-Yi Chen, Christopher S Hofmann, Bryan J Cottrell, Terence P Strobaugh, George C Paoli, Ly-Huong Nguyen, Xianghe Yan, Gaylen A Uhlich
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e84863 (2013)
The biofilm life style helps bacteria resist oxidative stress, desiccation, antibiotic treatment, and starvation. Biofilm formation involves a complex regulatory gene network controlled by various environmental signals. It was previously shown that p
Externí odkaz:
https://doaj.org/article/6469a6ac2a464572b5e6f84e93b92e7c
Publikováno v:
Appl Environ Microbiol
Prophage-encoded Escherichia coli O157:H7 transcription factor (TF), PchE, inhibits biofilm formation and attachment to cultured epithelial cells by reducing curli fimbriae expression and increasing flagella expression. To identify pchE regulators th
Publikováno v:
Microbiology Resource Announcements
Campylobacter strains were collected in a survey of fresh chicken carcasses in Pennsylvania farmers’ markets. Three Campylobacter coli strains were observed to have unique sequence variations in their gyrase subunit B genes, compared with other Cam
Publikováno v:
Microbiology Resource Announcements
Escherichia coli serotype O157:H7 strain 20R2R is a derivative of clinical isolate PA20. Prophage excision from the coding region of a PA20 transcription factor restored RpoS-dependent biofilm formation in 20R2R, providing a model for O157:H7 stress
Autor:
Elisa, Andreozzi, Gaylen A, Uhlich
Publikováno v:
International Journal of Molecular Sciences
Shiga toxins and intimate adhesion controlled by the locus of enterocyte effacement are major enterohemorrhagic Escherichia coli (EHEC) virulence factors. Curli fimbriae also contribute to cell adhesion and are essential biofilm components. The trans
Autor:
Peter L. Irwin, Ly-Huong T. Nguyen, Elisa Andreozzi, Gaylen A. Uhlich, Bryan J. Cottrell, Chin-Yi Chen
Publikováno v:
Microbiology. 163:611-621
Expression of the major biofilm components of E. coli, curli fimbriae and cellulose, requires the CsgD transcription factor. A complex regulatory network allows environmental control of csgD transcription and biofilm formation. However, most clinical
Publikováno v:
Microbiology Resource Announcements
Escherichia coli serotype O157:H7 strain ATCC 43888 is a Shiga toxin-deficient human fecal isolate. Due to its reduced toxicity and its availability from a curated culture collection, the strain has been used extensively in applied research studies.