Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Gavain Sweetman"'
Autor:
Toby Mathieson, Holger Franken, Jan Kosinski, Nils Kurzawa, Nico Zinn, Gavain Sweetman, Daniel Poeckel, Vikram S. Ratnu, Maike Schramm, Isabelle Becher, Michael Steidel, Kyung-Min Noh, Giovanna Bergamini, Martin Beck, Marcus Bantscheff, Mikhail M. Savitski
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
The proteome-wide characterization of proteostasis depends on robust approaches to determine protein half-lives. Here, the authors improve the accuracy and precision of mass spectrometry-based quantification, enabling reliable protein half-life deter
Externí odkaz:
https://doaj.org/article/da0726d0c6b44f4fa437f686bacfcd20
Autor:
Carola Doce, Christian Fufezan, Marcus Bantscheff, Nico Zinn, Christine Boecker, Toby Mathieson, Thilo Werner, Gavain Sweetman
Publikováno v:
Journal of proteome research. 20(3)
Multiplexed quantitative proteomics enabled complex workflows to study the mechanisms by which small molecule drugs interact with the proteome such as thermal proteome profiling (TPP) or multiplexed proteome dynamics profiling (mPDP). TPP measures ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d356ea7731030a4d4bad0a79b52cf30c
https://pubmed.ncbi.nlm.nih.gov/33621079
https://pubmed.ncbi.nlm.nih.gov/33621079
Autor:
Isabelle Becher, Daniel Poeckel, Maike Schramm, Kyung-Min Noh, Michael Steidel, Mikhail M. Savitski, Vikram S. Ratnu, Gavain Sweetman, Giovanna Bergamini, Holger Franken, Nils Kurzawa, Martin Beck, Marcus Bantscheff, Nico Zinn, Toby Mathieson, Jan Kosinski
Publikováno v:
Nature Communications
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
Nature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
A better understanding of proteostasis in health and disease requires robust methods to determine protein half-lives. Here we improve the precision and accuracy of peptide ion intensity-based quantification, enabling more accurate protein turnover de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18af357a7c2cb379e8ec8ed90ae99d18
https://pubmed.ncbi.nlm.nih.gov/29449567
https://pubmed.ncbi.nlm.nih.gov/29449567
Autor:
Thilo Werner, Isabelle Becher, Mikhail M. Savitski, Marcus Bantscheff, Gavain Sweetman, Carola Doce
Publikováno v:
Analytical Chemistry. 84:7188-7194
Isobaric mass tag-based quantitative proteomics strategies such as iTRAQ and TMT utilize reporter ions in the low-mass range of tandem MS spectra for relative quantification. The number of samples that can be compared in a single experiment (multiple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a65158823d8d79fc2434c91c66357a2
https://doi.org/10.1021/ac301553x
https://doi.org/10.1021/ac301553x
Publikováno v:
Analytical Chemistry. 82:9843-9849
Here we describe a set of enhanced data processing and filtering methods to improve significance and coverage of phosphopeptide identifications by mass spectrometry. We demonstrate that for samples of limited complexity, spectra-based estimation of f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c675a7ffd79e130c701d27516453f29b
https://doi.org/10.1021/ac102083q
https://doi.org/10.1021/ac102083q
Autor:
Gerard Drewes, Andreas Bauer, Toby Mathieson, Bernhard Kuster, Gitte Neubauer, Yann Abraham, Marcus Bantscheff, Carsten Hopf, Manfred Raida, Scott Hobson, Gavain Sweetman, Ulrich Kruse, Jens Rick, Nigel Ramsden, Tewis Bouwmeester, Sonja Bastuck, Valerie Reader, Dirk Eberhard, Jessica Perrin, Markus Boesche, Christina Rau
Publikováno v:
Nature Biotechnology. 25:1035-1044
We describe a chemical proteomics approach to profile the interaction of small molecules with hundreds of endogenously expressed protein kinases and purine-binding proteins. This subproteome is captured by immobilized nonselective kinase inhibitors (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f04550d4646c3a3d6c190b8e33a18b6
https://doi.org/10.1038/nbt1328
https://doi.org/10.1038/nbt1328
Autor:
Marcus Bantscheff, Ina Tögel, Holger Franken, Thilo Werner, Wolfgang Huber, Dorothee Childs, Gerard Drewes, Stephan Gade, Toby Mathieson, Carola Doce, Gavain Sweetman, Mikhail M. Savitski, Friedrich B M Reinhard
Publikováno v:
Nature protocols. 10(10)
The direct detection of drug-protein interactions in living cells is a major challenge in drug discovery research. Recently, we introduced an approach termed thermal proteome profiling (TPP), which enables the monitoring of changes in protein thermal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43b66295e225640bdc595031cda30c77
https://pubmed.ncbi.nlm.nih.gov/26379230
https://pubmed.ncbi.nlm.nih.gov/26379230
Autor:
Gavain Sweetman, Marcus Bantscheff, Toby Mathieson, Thilo Werner, Mikhail M. Savitski, Maria Fälth Savitski
Publikováno v:
Analytical chemistry. 86(7)
Isobaric mass tag-based quantitative proteomics strategies such as iTRAQ and TMT utilize reporter ions in the low mass range of tandem MS spectra for relative quantification. The recent extension of TMT multiplexing to 10 conditions has been enabled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b79ee2037639735a48ec22e72eb5666
https://pubmed.ncbi.nlm.nih.gov/24579773
https://pubmed.ncbi.nlm.nih.gov/24579773
Autor:
Marcus Bantscheff, Fiona Pachl, Isabelle Becher, Toby Mathieson, Nico Zinn, Bernhard Kuster, Mikhail M. Savitski, Carola Doce, Gavain Sweetman
Publikováno v:
Journal of proteome research. 12(8)
Isobaric mass tagging (e.g., TMT and iTRAQ) is a precise and sensitive multiplexed peptide/protein quantification technique in mass spectrometry. However, accurate quantification of complex proteomic samples is impaired by cofragmentation of peptides
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90e2c32804334bd928daf473b9b4db07
https://pubmed.ncbi.nlm.nih.gov/23768245
https://pubmed.ncbi.nlm.nih.gov/23768245
Autor:
Marcus Bantscheff, Jarrod A. Marto, Nico Zinn, Manor Askenazi, Mikhail M. Savitski, Manja Lang, Gavain Sweetman
Publikováno v:
Analytical chemistry. 83(23)
Fragmentation of multiple peptides in a single tandem mass scan impairs accuracy of isobaric mass tag based quantification. Consequently, practitioners aim at fragmenting peptide ions with the highest possible purity without compromising on sensitivi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28b48cc3b9f8dfe4045e4cc8da940886
https://pubmed.ncbi.nlm.nih.gov/22017476
https://pubmed.ncbi.nlm.nih.gov/22017476