A Hypersensitive Genetically Encoded Fluorescent Indicator (roGFP2-Prx1) Enables Continuous Measurement of Intracellular H2O2 during Cell Micro-cultivation

Autor: Gast, Veronica, Siewers, Verena, Molin, Mikael

Publikováno v: Bio Protoc

Hydrogen peroxide (H(2)O(2)) is a toxic oxidant produced as a byproduct of several biological processes. At too high levels of hydrogen peroxide cells will experience oxidative stress, leading to a cellular response to decrease its levels and to prot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b25a80eb9a62805de6676bef5214c3c2
https://doi.org/10.21769/bioprotoc.4317

The Yeast eIF2 Kinase Gcn2 Facilitates H2O2-Mediated Feedback Inhibition of Both Protein Synthesis and Endoplasmic Reticulum Oxidative Folding during Recombinant Protein Production.

Autor: Gast, Veronica¹, Campbell, Kate^1,2, Picazo, Cecilia^1,3, Engqvist, Martin¹, Siewers, Verena^1,2, Molin, Mikael¹ Mikael.molin@chalmers.se

Publikováno v: Applied & Environmental Microbiology. Aug2021, Vol. 87 Issue 15, p1-16. 16p.

A Hypersensitive Genetically Encoded Fluorescent Indicator (roGFP2-Prx1) Enables Continuous Measurement of Intracellular H 2 O 2 during Cell Micro-cultivation.

Autor: Gast V; Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden., Siewers V; Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden., Molin M; Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.

Publikováno v: Bio-protocol [Bio Protoc] 2022 Feb 05; Vol. 12 (3), pp. e4317. Date of Electronic Publication: 2022 Feb 05 (Print Publication: 2022).