Impact of the T296S mutation in P450 GcoA for aryl-O-demethylation: a QM/MM study

Autor: Sonia F. G. Santos, Rajesh Reddy Bommareddy, Gary W. Black, Warispreet Singh

Publikováno v: Frontiers in Chemistry, Vol 11 (2024)

Lignin, a complex plant cell wall component, holds promise as a renewable aromatic carbon feedstock. p-Vanillin is a key product of lignin depolymerization and a precursor of protocatechuic acid (PCA) that has tremendous potential for biofuel product

Externí odkaz: https://doaj.org/article/f50af5321ea548dba33e6b7feff08e3e

In Search of Complementary Extraction Methods for Comprehensive Coverage of the Escherichia coli Metabolome

Autor: Henry Gould, William Cheung, James D. Finnigan, José Muñoz-Muñoz, Simon J. Charnock, Gary W. Black

Publikováno v: Metabolites, Vol 13, Iss 9, p 1010 (2023)

Escherichia coli is an invaluable research tool for many fields of biology, in particular for the production of recombinant enzymes. However, the activity of many such recombinant enzymes cannot be determined using standard biochemical assays, as oft

Externí odkaz: https://doaj.org/article/9100f8bd24d64e808d5ab900fc20a501

Biocatalytic and Chemo-Enzymatic Synthesis of Quinolines and 2-Quinolones by Monoamine Oxidase (MAO-N) and Horseradish Peroxidase (HRP) Biocatalysts

Autor: Haoyue Xiang, Salvatore Ferla, Carmine Varricchio, Andrea Brancale, Nicola L. Brown, Gary W. Black, Nicholas J. Turner, Daniele Castagnolo

Publikováno v: ACS Catalysis. 13:3370-3378

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::83e1a26867bb2f482d35b1c2e2d0f4f4
https://doi.org/10.1021/acscatal.2c05902

Single-Site Mutation Induces Water-Mediated Promiscuity in Lignin Breaking Cytochrome P450GcoA

Autor: Warispreet Singh, Sónia F. G. Santos, Paul James, Gary W. Black, Meilan Huang, Kshatresh Dutta Dubey

Publikováno v: Singh, W, Santos, S F G, James, P, Black, G W, Huang, M & Dubey, K D 2022, ' Single-Site Mutation Induces Water-Mediated Promiscuity in Lignin Breaking Cytochrome P450GcoA ', ACS Omega . https://doi.org/10.1021/acsomega.2c00524

Cytochrome P450 GcoA is an enzyme that catalyses the guaiacol unit of lignin during the lignin breakdown via aryl-O-demethylation reaction. This reaction is intriguing and is of commercial importance for its potential application in the production of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0c4d0db2b665e3c68de0523335cdead
https://doi.org/10.1021/acsomega.2c00524

A facile and regioselective multicomponent synthesis of chiral aryl-1,2-mercaptoamines in water followed by monoamine oxidase (MAO-N) enzymatic resolution

Autor: Kate Lauder, Gary W. Black, Nicholas J. Turner, Aya Al Mekdad, Renzo Luisi, Daniele Castagnolo, Anita Toscani, Nicola Brown, Domiziana Masci

Publikováno v: Organic & Biomolecular Chemistry. 17:8982-8986

A facile microwave assisted three-component protocol allows the synthesis of chiral aryl-1,2-mercaptoamines in water in a few minutes with high yields, bypassing the use of toxic aziridine intermediates. The chiral 1,2-mercaptoamines were then derace

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70016530d2392ee797523f1120fce5be
https://doi.org/10.1039/c9ob01962f