Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Gary S. Koe"'
Compositional effects of cationic lipid/DNA delivery systems on transgene expression in cell culture
Publikováno v:
Journal of Pharmaceutical Sciences. 93:108-123
Studies of the contribution of various physical properties of cationic lipid/DNA complexes (CLDCs) to their observed transgene expression in vitro were conducted using cationic liposomes composed of the cationic lipids 1,2‐dioleoyl‐3‐trimethyla
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58516ee229d659dd815234b19a59b0bf
https://doi.org/10.1002/jps.10519
https://doi.org/10.1002/jps.10519
Autor:
Sirirat Choosakoonkriang, Janet G. Smith, Gouri S. Jas, C. Russell Middaugh, Gary S. Koe, Chad S. Braun
Publikováno v:
Biophysical Journal. 84(2):1114-1123
The structure of DNA within CLDCs used for gene delivery is controversial. Previous studies using CD have been interpreted to indicate that the DNA is converted from normal B to C form in complexes. This investigation reexamines this interpretation u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fff326969c90bf7edc3a7f5539d5cca7
Publikováno v:
Journal of Biological Chemistry. 276:32806-32813
Recent evidence supports a role for proteoglycans in polycation-mediated gene delivery. Therefore, the interaction of glycosaminoglycans with cationic lipid-DNA complexes (CLDCs) has been characterized using a combination of biophysical approaches. A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2cf60766ff5638f24a3f5f0e426db951
https://doi.org/10.1074/jbc.m007940200
https://doi.org/10.1074/jbc.m007940200
Autor:
C. Russell Middaugh, Gary S. Koe, Janet G. Smith, Christopher M. Wiethoff, Thomas J. Anchordoquy, Sirirat Choosakoonkriang
Publikováno v:
Journal of Biological Chemistry. 276:8037-8043
Fourier transform infrared spectroscopy was used to characterize the interaction of the cationic lipids 1,2-dioleoyl-3-trimethylammonium-propane and dioctadecyldimethylammonium bromide with plasmid DNA. The effect of incorporating the neutral colipid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7466771496038e76e49b109e3678ccdb
https://doi.org/10.1074/jbc.m010592200
https://doi.org/10.1074/jbc.m010592200
Autor:
Gary S. Koe, Thomas J. Anchordoquy
Publikováno v:
Journal of Pharmaceutical Sciences. 89:289-296
Nonviral, plasmid-based therapeutics are a new class of pharmaceutical agents that offer the potential to cure many diseases that are currently considered untreatable. While nonviral vectors have shown promise in clinical trials, their physical insta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::27ee61fccd0ca47d914193faeb6e5761
https://doi.org/10.1002/(sici)1520-6017(200003)89:3<289::aid-jps1>3.0.co
https://doi.org/10.1002/(sici)1520-6017(200003)89:3<289::aid-jps1>3.0.co
Autor:
Thomas A. Bewley, Chung C. Hsu, Dennis Brooks, Rodney Pearlman, Hoc M. Nguyen, Gary S. Koe, Douglas A. Yeung
Publikováno v:
Pharmaceutical Research. 12:69-77
During protein lyophilization, it is common practice to complete the freezing step as fast as possible in order to avoid protein denaturation, as well as to obtain a final product of uniform quality. We report a contradictory observation made during
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7b06e4bfc63b416742db6b9112536009
https://doi.org/10.1023/a:1016270103863
https://doi.org/10.1023/a:1016270103863
Autor:
Vincent L. Vilker, Gary S. Koe
Publikováno v:
Biotechnology Progress. 9:608-614
A P-450cam monooxygenase enzyme system, obtained through separate purification of the three protein subunits, was used as a biocatalyst in the dehalogenation of 1,2-dibromo-3-chloropropane (DBCP). Incubations in a solution containing the reconstitute
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e03c99bfeaebb8921532b28b046449ec
https://doi.org/10.1021/bp00024a007
https://doi.org/10.1021/bp00024a007
Effect of oxygen on the dehalogenation of 1,2-Dibromo-3-chloropropane by cytochrome P450cam (CYP101)
Autor:
Vincent L. Vilker, Gary S. Koe
Publikováno v:
Biotechnology progress. 21(4)
Cytochromes P450 are known to exhibit diverse catalytic functions against a large number of hydrocarbon substrates. The determinants of specific activity(ies) that operate on specific substrates have not been widely explored. Earlier, we showed that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0713aae26b34f31522b63cf7b7e10282
https://pubmed.ncbi.nlm.nih.gov/16080691
https://pubmed.ncbi.nlm.nih.gov/16080691
Autor:
Chad S. Braun, Gary S. Koe, Donald A. Tomalia, Janet G. Koe, C. Russell Middaugh, Mark T. Fisher
Publikováno v:
Biophysical journal. 88(6)
Stopped-flow circular dichroism and fluorescence spectroscopy are used to characterize the assembly of complexes consisting of plasmid DNA bound to the cationic lipids dimethyldioctadecylammonium bromide and 1, 2-dioleoyl- 3-trimethylammonium-propane
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7659a73883d7930753b789b9befafcc5
https://pubmed.ncbi.nlm.nih.gov/15805171
https://pubmed.ncbi.nlm.nih.gov/15805171
Autor:
Gary S. Koe, Joseph A. Vetro, C. Russell Middaugh, Donald A. Tomalia, Chad S. Braun, Janet G. Koe
Publikováno v:
Journal of pharmaceutical sciences. 94(2)
PAMAM dendrimers are members of a class of polyamine polymers that demonstrate significant gene delivery ability. In this study, a selection of PAMAM dendrimers, spanning a range of sizes (generations 2, 4, 7, and 9) and transfection efficiencies, ar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1c6af86a445d85f9b3a3f89450de05b
https://pubmed.ncbi.nlm.nih.gov/15614818
https://pubmed.ncbi.nlm.nih.gov/15614818