Zobrazeno 1 - 10
of 94
pro vyhledávání: '"Gary R. Matsueda"'
Publikováno v:
International Journal of Peptide and Protein Research. 28:107-112
The 3-nitro-2-pyridinesulfenyl (Npys) derivative of cysteine was prepared and used to facilitate the formation of an unsymmetrical disulfide bond. Since this derivative is stable in trifluoroacetic acid:CH2 Cl2 (1:1) and anhydrous hydrogen fluoride,
Autor:
Gary R. Matsueda
Publikováno v:
International Journal of Peptide and Protein Research. 20:26-34
Deprotection of Nin-formyl tryptophan (Trp) occurs during liquid hydrogen fluoride treatment at 0 degrees when 1,2-ethanedithiol (EDT), or 1,4-butanedithiol, is present. Deformylation, as evidenced by amino acid analysis and ultraviolet spectral anal
Autor:
Gary R. Matsueda, Stephan A. Gaehde
Publikováno v:
International Journal of Peptide and Protein Research. 18:451-458
N-tert.-butoxycarbonyl-aminomethyl( alpha-phenyl)phenoxyacetic acid was synthesized and found to be suitable for use as a handle in the solid-phase synthesis of peptide alpha-carboxamides. This handle was prepared with an 82% yield when N-tert.-butox
Publikováno v:
International Journal of Peptide and Protein Research. 19:490-498
The 3-nitro-2-pyridinesulfenyl (Npys) group has been used successfully for side chain protection of cysteine during the stepwise solid-phase synthesis of Lys8-vasopressin (LVP) on benzhydrylamine resin. The versatility and limitations of this group h
Publikováno v:
Thrombosis and Haemostasis. 76:429-438
SummaryChimeric 59D8-SK was designed to confer fibrin-selectivity to streptokinase by fusion of the Fab fragment of anti-fibrin antibody 59D8 to the N-terminus of streptokinase (SK: Ile1-Lys414). It was expressed in a mouse hybridoma cell line and pu
Autor:
Hann-Guang Chao, Gary R. Matsueda, Thomas B. Lavoie, Liyang Shen, Jiri Novotny, Robert Fairman, Luciano Mueller
Publikováno v:
Protein Science. 4:1457-1469
Limited information is available on inherent stabilities of four-chain-coils. We have developed a model system to study this folding motif using synthetic peptides derived from sequences contained in the tetramerization domain of Lac repressor. These
Publikováno v:
Journal of Biological Chemistry. 270:11590-11594
Syk is a cytoplasmic protein-tyrosine kinase containing two amino-terminal Src homology 2 domains that is activated following ligation of the B cell antigen receptor. Syk activation in B cells correlates with Syk tyrosine phosphorylation as well as w
Publikováno v:
Analytical Biochemistry. 223:88-92
Efforts to develop an improved assay for plasma and tissue transglutaminase have led us to a convenient, sensitive microtiter plate assay for coagulation factor XIII using human fibrinogen as an immobilized substrate. Factor XIII was activated in the
Publikováno v:
The Journal of Organic Chemistry. 59:6687-6691
Autor:
K.D. Michelson, Marschall S. Runge, Edgar Haber, Ted W. Love, Thomas Quertermous, Gary R. Matsueda
Publikováno v:
Fibrinolysis. 8:326-332
On evidence that the potency and specificity of tissue-type plasminogen activator (t-PA) can be improved by chemical conjugation to an antifibrin antibody (59D8), we constructed a recombinant molecule, 59D8-t-PA(AB), containing a truncated 59D8 antib