Zobrazeno 1 - 10 of 203 pro vyhledávání: '"Gary J. Pielak"'
1
Akademický článek
Using NMR-detected hydrogen-deuterium exchange to quantify protein stability in cosolutes, under crowded conditions in vitro and in cells
Autor: I-Te Chu, Gary J. Pielak
Publikováno v: Magnetic Resonance Letters, Vol 3, Iss 4, Pp 319-326 (2023)
We review the use of nuclear magnetic resonance (NMR) spectroscopy to assess the exchange of amide protons for deuterons (HDX) in efforts to understand how high concentration of cosolutes, especially macromolecules, affect the equilibrium thermodynam
Externí odkaz: https://doaj.org/article/d27e0da3830b408eb7853b2cb02aa532
Zobrazit plný text záznamu
2
Akademický článek
C-terminal truncation modulates α-Synuclein’s cytotoxicity and aggregation by promoting the interactions with membrane and chaperone
Autor: Cai Zhang, Yunshan Pei, Zeting Zhang, Lingling Xu, Xiaoli Liu, Ling Jiang, Gary J. Pielak, Xin Zhou, Maili Liu, Conggang Li
Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-10 (2022)
C-terminal truncation of a-syn results in a more extended and exposed conformation, providing further insight into the pathological role of this truncation event in the progression of Parkinson’s disease.
Externí odkaz: https://doaj.org/article/1cfdf1e867234e4aacfca93441cf8998
Zobrazit plný text záznamu
3
Akademický článek
Toxicity and Immunogenicity of a Tardigrade Cytosolic Abundant Heat Soluble Protein in Mice
Autor: Harrison J. Esterly, Candice J. Crilly, Samantha Piszkiewicz, Dane J. Shovlin, Gary J. Pielak, Brooke E. Christian
Publikováno v: Frontiers in Pharmacology, Vol 11 (2020)
Tardigrades are microscopic animals well-known for their stress tolerance, including the ability to survive desiccation. This survival requires cytosolic abundant heat soluble (CAHS) proteins. CAHS D protects enzymes from desiccation- and lyophilizat
Externí odkaz: https://doaj.org/article/5b810ebc53fa45d5b6788e5010033736
Zobrazit plný text záznamu
8
Dynamical spectroscopy and microscopy of proteins in cells
Autor: Martin Gruebele, Gary J. Pielak
Publikováno v: Current Opinion in Structural Biology. 70:1-7
With a strong understanding of how proteins fold in hand, it is now possible to ask how in-cell environments modulate their folding, binding and function. Studies accessing fast (ns to s) in-cell dynamics have accelerated over the past few years thro
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::219771452e325d76753c3780c9232323
https://doi.org/10.1016/j.sbi.2021.02.001
Zobrazit plný text záznamu
9
Secondary structure and stability of a gel-forming tardigrade desiccation-tolerance protein
Autor: Jonathan E. Eicher, Julia A. Brom, Shikun Wang, Sergei S. Sheiko, Joanna M. Atkin, Gary J. Pielak
Publikováno v: Protein science : a publication of the Protein Society. 31(12)
Protein-based pharmaceuticals are increasingly important, but their inherent instability necessitates a "cold chain" requiring costly refrigeration during production, shipment, and storage. Drying can overcome this problem, but most proteins need the
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::348c4d45fb6eda9e855615fae0f29d0c
https://pubmed.ncbi.nlm.nih.gov/36335581
Zobrazit plný text záznamu
10
Buffers, Especially the Good Kind
Autor: Gary J. Pielak
Publikováno v: Biochemistry. 60:3436-3440
Fifty-five years ago, Norman Good and colleagues authored a paper that fundamentally advanced wet biochemistry [Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., and Singh, R. M. M. (1966) Hydrogen ion buffers for biological researc
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32288a39afc49e9d6490e6288ca53ae6
https://doi.org/10.1021/acs.biochem.1c00200
Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání

Omezení vyhledávání
Plný text Recenzováno Digitální knihovna AV ČR
Zdroje