Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Gary F. Gerard"'
Autor:
Peter Qiu, Harini Shandilya, James M. D'Alessio, Kevin O'Connor, Jeffrey Durocher, Gary F. Gerard
Publikováno v:
BioTechniques, Vol 36, Iss 4, Pp 702-707 (2004)
We have developed a simple and flexible mutation detection technology for the discovery and mapping of both known and unknown mutations. This technology is based on a new mismatch-specific DNA endonuclease from celery, Surveyor™ nuclease, which is
Externí odkaz:
https://doaj.org/article/bbd7e094ae3a4c958ed783017e230139
Publikováno v:
BioTechniques, Vol 33, Iss 5, Pp 984-990 (2002)
Externí odkaz:
https://doaj.org/article/a4565b95e8bd4501b327713011166d1d
Autor:
Lee E. Moore, Frederic M. Waldman, Wong-Ho Chow, Nathaniel Rothman, Paul Brennan, Paolo Boffetta, Berton Zbar, Maria Merino, W. Marston Linehan, Gary F. Gerard, Rayjean Hung, Sara Karami, Jorge R. Toro, Laura S. Schmidt, Ivana Holcatova, Anush Mukeria, Dana Mates, Neonilia Szeszenia-Dabrowska, Marie Navratilova, Vladimir Bencko, Hellena Kollarova, Vladimir Janout, Vsevolod Matveev, David Zaridze, Sunil Mahurkar, Jeffrey A. Durocher, Yangu Shi, Erich Jaeger, Michael L. Nickerson
Purpose: To provide a comprehensive, thorough analysis of somatic mutation and promoter hypermethylation of the von Hippel-Lindau (VHL) gene in the cancer genome, unique to clear cell renal cancer (ccRCC). Identify relationships between the prevalenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc75ebcaa169f5a7041e2df9441c11dc
https://doi.org/10.1158/1078-0432.c.6517150
https://doi.org/10.1158/1078-0432.c.6517150
Autor:
Gary F. Gerard
Publikováno v:
Enzymes of Nucleic Acid Synthesis and Modification ISBN: 9781351071857
Retrovirus reverse transcriptase has been the subject of intense investigation since its discovery in 1970. Retroviruses and their resident reverse transcriptases have been characterized from many different vertebrates. In order to understand the rol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5417b0525fe09496f2629b9d74e065a2
https://doi.org/10.1201/9781351071857-1
https://doi.org/10.1201/9781351071857-1
Autor:
Gary F. Gerard, Eric Kaldjian, Ashley Larrimore, Kristen P. Nickens, Ying Han, Steven R. Patierno, Harini Shandilya, Susan Ceryak
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1823(2):264-272
Acquisition of death-resistance is critical in the evolution of neoplasia. Our aim was to model the early stages of carcinogenesis by examining intracellular alterations in cells that have acquired apoptosis-resistance after exposure to a complex gen
Publikováno v:
Molecular Biotechnology. 29:11-18
Site-directed mutagenesis and polymerase chain reaction (PCR)-based cloning are well-established methods carried out routinely in most modern molecular biology laboratories. Application of these methods requires confirmation of the DNA sequence of th
Autor:
Moreland D. Gibbs, Mekbib Germantown Astatke, Peter L. Bergquist, Po-Jen Shih, Elizabeth Flynn, Harini Shandilya, Jun E. Lee, Katherine R. Griffiths, Gary F. Gerard
Publikováno v:
Extremophiles. 8:243-251
Conserved motifs found in known bacterial polI DNA polymerase sequences were identified, and degenerate PCR primers were designed for PCR amplification of an internal portion of polI genes from all bacterial divisions. We describe here a method that
Autor:
James M. D'Alessio, Harini Shandilya, Peter Qiu, Kevin C. O’Connor, Jeffrey A. Durocher, Gary F. Gerard
Publikováno v:
BioTechniques. 36:702-707
We have developed a simple and flexible mutation detection technology for the discovery and mapping of both known and unknown mutations. This technology is based on a new mismatch-specific DNA endonuclease from celery, Surveyor™ nuclease, which is
Autor:
Deb K. Chatterjee, Gary F. Gerard, Michael D. Smith, R. Jason Potter, Kim Rosenthal, Gulshan Dhariwal, Jun Lee
Publikováno v:
Nucleic Acids Research. 30:3118-3129
We compared the thermal stabilities of wild-type recombinant avian myeloblastosis virus (AMV) and Moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) with those of mutants of the recombinant enzymes lacking RNase H activity. They differe
Publikováno v:
Molecular Biotechnology. 8:61-77
Reverse transcriptase (RT) is the key enzyme required for conversion of RNA to DNA. Cloning of Moloney murine leukemia virus (MMLV) RT has enable engineering an RT that lacks endogenous RNase H activity. RT catalyzes cDNA synthesis more efficiently i