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Akademický článek

Morph-X-Select: Morphology-based tissue aptamer selection for ovarian cancer biomarker discovery

Autor: Hongyu Wang, Xin Li, David E. Volk, Ganesh L.-R. Lokesh, Miguel-Angel Elizondo-Riojas, Li Li, Alpa M. Nick, Anil K. Sood, Kevin P. Rosenblatt, David G. Gorenstein

Publikováno v: BioTechniques, Vol 61, Iss 5, Pp 249-259 (2016)

High affinity aptamer-based biomarker discovery has the advantage of simultaneously discovering an aptamer affinity reagent and its target biomarker protein. Here, we demonstrate a morphology-based tissue aptamer selection method that enables us to u

Externí odkaz: https://doaj.org/article/24d2a00ff8614c07a1e8e263ccbc4ccb

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Akademický článek

A Unique and Simple Approach to Improve Sensitivity in 15N-NMR Relaxation Measurements for NH3+ Groups: Application to a Protein-DNA Complex

Autor: Dan Nguyen, Ganesh L. R. Lokesh, David E. Volk, Junji Iwahara

Publikováno v: Molecules, Vol 22, Iss 8, p 1355 (2017)

NMR spectroscopy is a powerful tool for research on protein dynamics. In the past decade, there has been significant progress in the development of NMR methods for studying charged side chains. In particular, NMR methods for lysine side-chain NH3+ gr

Externí odkaz: https://doaj.org/article/bc06502691634ff4b87898b710cfa566

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Akademický článek

Development of Phosphorothioate DNA and DNA Thioaptamers

Autor: David E. Volk, Ganesh L. R. Lokesh

Publikováno v: Biomedicines, Vol 5, Iss 3, p 41 (2017)

Nucleic acid aptamers are short RNA- or DNA-based affinity reagents typically selected from combinatorial libraries to bind to a specific target such as a protein, a small molecule, whole cells or even animals. Aptamers have utility in the developmen

Externí odkaz: https://doaj.org/article/f73d80b58c954bd39a7b3b67c0f71b80

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