Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Gale E. Smith"'
Publikováno v:
Virology. 131:561-565
The nucleotide sequence of a 1143-bp region of Autographa californica MNPV (AcMNPV) DNA containing the entire coding region of polyhedrin was determined. The coding region consisted of 732 bp without intervening sequences. The 5′ leader sequence wa
Publikováno v:
Journal of Invertebrate Pathology. 42:299-305
A biochemical and limited morphological characterization of an entomopoxvirus infecting the lesser cornstalk borer, Elasmopalpus lignosellus , was made. The oval virions measure 270 × 200 nm and the spheroids average 1.5 μm in diameter. Sodium dode
Publikováno v:
Journal of Virology. 45:215-225
In Autographa californica nuclear polyhedrosis virus-infected cells, polyhedrin, the major structural polypeptide of the viral occlusions, and a low-molecular-weight viral protein with a molecular weight of approximately 10,000 (10K) accumulated to h
Autor:
Max D. Summers, Hans-Werner Lahm, Gale E. Smith, Grace Ju, Brad L. Ericson, Richard Anthony Chizzonite, John Moschera
Publikováno v:
Proceedings of the National Academy of Sciences. 82:8404-8408
A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypepti
Autor:
Max D. Summers, Gale E. Smith
Publikováno v:
Analytical Biochemistry. 109:123-129
A method, called “bidirectional transfer”, has been described for the transfer of DNA and RNA from agarose or polyacrylamide gels onto diazobenzyloxymethyl (DBM)-paper or nitrocellulose filters. The gels were sandwiched between either two nitroce
Publikováno v:
Molecular and Cellular Biology. 5:2860-2865
A cDNA fragment coding for human c-myc was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. Insect cells infected with the recombinant virus produced significant
Autor:
Gale E. Smith, Max D. Summers
Publikováno v:
Virology. 84:390-402
The structural polypeptides of eight insect baculoviruses were studied using vertical slab polyacrylamide gel electrophoresis. All viruses revealed a complex but unique composition of 15 to 25 bands with molecular weights ranging from 15,000 to 160,0
Autor:
Gale E. Smith, Max D. Summers
Publikováno v:
Journal of Virology. 30:828-838
The restriction sites of Autographa californica nuclear polyhedrosis virus (Ac M NPV) E2 DNA were mapped for the endonucleases Sma I, Kpn I, Bam HI, Sac I, Xho I, and Eco RI. The restriction maps of four other Ac M NPV variants, Trichoplusia ni (Tn M
Publikováno v:
Journal of Virology. 46:584-593
We describe a method to introduce site-specific mutations into the genome of Autographa californica nuclear polyhedrosis virus. Specifically, the A. californica nuclear polyhedrosis virus gene for polyhedrin, the major protein that forms viral occlus
Publikováno v:
Journal of Invertebrate Pathology. 30:362-368
A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera fr