Increasing the bulk of the 1TEL-target linker and retaining the 10×His tag in a 1TEL-CMG2-vWa construct improves crystal order and diffraction limits.

Autor: Gajjar PL; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Pedroza Romo MJ; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Litchfield CM; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Callahan M; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Redd N; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Nawarathnage S; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Soleimani S; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Averett J; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Wilson E; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Lewis A; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Stewart C; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Tseng YJ; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA., Doukov T; Macromolecular Crystallography Group, Structural Molecular Biology Resource, Stanford Synchrotron Radiation Lightsource, Menlo Park, California, USA., Lebedev A; Scientific Computing, STFC Rutherford Appleton Laboratory, Didcot OX11 0QX, United Kingdom., Moody JD; Department of Chemistry and Biochemistry, Brigham Young University, 701 East University Parkway, Provo, UT 84602, USA.

Publikováno v: Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2023 Oct 01; Vol. 79 (Pt 10), pp. 925-943. Date of Electronic Publication: 2023 Sep 25.