Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization.

Autor: Bell EL; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Rosetto G; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA., Ingraham MA; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Ramirez KJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Lincoln C; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Clarke RW; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Gado JE; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Lilly JL; Battelle Memorial Institute, Columbus, OH, 43201, USA., Kucharzyk KH; Battelle Memorial Institute, Columbus, OH, 43201, USA., Erickson E; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA.; BOTTLE Consortium, Golden, CO, 80401, USA., Beckham GT; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, 80401, USA. gregg.beckham@nrel.gov.; BOTTLE Consortium, Golden, CO, 80401, USA. gregg.beckham@nrel.gov.

Publikováno v: Nature communications [Nat Commun] 2024 Feb 09; Vol. 15 (1), pp. 1217. Date of Electronic Publication: 2024 Feb 09.

Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity.

Autor: Erickson E; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.; BOTTLE Consortium, Golden, CO, USA., Gado JE; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.; BOTTLE Consortium, Golden, CO, USA., Avilán L; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Bratti F; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.; BOTTLE Consortium, Golden, CO, USA., Brizendine RK; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.; BOTTLE Consortium, Golden, CO, USA., Cox PA; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Gill R; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Graham R; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Kim DJ; BOTTLE Consortium, Golden, CO, USA.; Department of Biochemistry, Montana State University, Bozeman, MT, USA., König G; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Michener WE; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.; BOTTLE Consortium, Golden, CO, USA., Poudel S; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Ramirez KJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA.; BOTTLE Consortium, Golden, CO, USA., Shakespeare TJ; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Zahn M; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Boyd ES; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Payne CM; National Science Foundation, Alexandria, VA, USA., DuBois JL; BOTTLE Consortium, Golden, CO, USA.; Department of Biochemistry, Montana State University, Bozeman, MT, USA., Pickford AR; BOTTLE Consortium, Golden, CO, USA.; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK., Beckham GT; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA. gregg.beckham@nrel.gov.; BOTTLE Consortium, Golden, CO, USA. gregg.beckham@nrel.gov., McGeehan JE; BOTTLE Consortium, Golden, CO, USA. drjohnmcgeehan@gmail.com.; Centre for Enzyme Innovation, School of Biological Sciences, University of Portsmouth, Portsmouth, UK. drjohnmcgeehan@gmail.com.; World Plastics Association, Fontvieille, Monaco. drjohnmcgeehan@gmail.com.

Publikováno v: Nature communications [Nat Commun] 2022 Dec 21; Vol. 13 (1), pp. 7850. Date of Electronic Publication: 2022 Dec 21.

Discovery of alkaline laccases from basidiomycete fungi through machine learning-based approach.

Autor: Wan, Xing¹ (AUTHOR) xing.wan@helsinki.fi, Shahrear, Sazzad¹ (AUTHOR), Chew, Shea Wen¹ (AUTHOR), Vilaplana, Francisco² (AUTHOR), Mäkelä, Miia R.^1,3 (AUTHOR) miia.makela@aalto.fi

Publikováno v: Biotechnology for Biofuels & Bioproducts. 9/11/2024, Vol. 17 Issue 1, p1-15. 15p.

Characterization and engineering of a two-enzyme system for plastics depolymerization.

Autor: Knott BC; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Erickson E; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Allen MD; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom., Gado JE; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401.; Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506., Graham R; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom., Kearns FL; Department of Chemistry, University of South Florida, Tampa, FL 33620., Pardo I; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Topuzlu E; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401.; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717., Anderson JJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Austin HP; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom., Dominick G; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Johnson CW; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Rorrer NA; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Szostkiewicz CJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Copié V; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717., Payne CM; Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY 40506., Woodcock HL; Department of Chemistry, University of South Florida, Tampa, FL 33620., Donohoe BS; Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Beckham GT; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO 80401; gregg.beckham@nrel.gov john.mcgeehan@port.ac.uk., McGeehan JE; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2DY, United Kingdom; gregg.beckham@nrel.gov john.mcgeehan@port.ac.uk.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Oct 13; Vol. 117 (41), pp. 25476-25485. Date of Electronic Publication: 2020 Sep 28.

Enabling microbial syringol conversion through structure-guided protein engineering.

Autor: Machovina MM; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717., Mallinson SJB; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom., Knott BC; Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Meyers AW; National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401., Garcia-Borràs M; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095., Bu L; Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Gado JE; National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401.; Department of Chemical Engineering, University of Kentucky, Lexington, KY 40506., Oliver A; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717., Schmidt GP; Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Hinchen DJ; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom., Crowley MF; Biosciences Center, National Renewable Energy Laboratory, Golden, CO 80401., Johnson CW; National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401., Neidle EL; Department of Microbiology, University of Georgia, Athens, GA 30602., Payne CM; Department of Chemical Engineering, University of Kentucky, Lexington, KY 40506., Houk KN; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095; houk@chem.ucla.edu gregg.beckham@nrel.gov john.mcgeehan@port.ac.uk jennifer.dubois1@montana.edu., Beckham GT; National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401; houk@chem.ucla.edu gregg.beckham@nrel.gov john.mcgeehan@port.ac.uk jennifer.dubois1@montana.edu.; Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37830., McGeehan JE; Centre for Enzyme Innovation, School of Biological Sciences, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom; houk@chem.ucla.edu gregg.beckham@nrel.gov john.mcgeehan@port.ac.uk jennifer.dubois1@montana.edu., DuBois JL; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717; houk@chem.ucla.edu gregg.beckham@nrel.gov john.mcgeehan@port.ac.uk jennifer.dubois1@montana.edu.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Jul 09; Vol. 116 (28), pp. 13970-13976. Date of Electronic Publication: 2019 Jun 24.