Kinetics of recruitment and allosteric activation of ARHGEF25 isoforms by the heterotrimeric G-protein Gαq.

Autor: van Unen J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands., Yin T; Center for Cell Analysis and Modeling, University of Connecticut Health Center, 400 Farmington Avenue, Farmington, CT 06032-6406, USA., Wu YI; Center for Cell Analysis and Modeling, University of Connecticut Health Center, 400 Farmington Avenue, Farmington, CT 06032-6406, USA., Mastop M; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands., Gadella TW Jr; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands., Goedhart J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands.

Publikováno v: Scientific reports [Sci Rep] 2016 Nov 11; Vol. 6, pp. 36825. Date of Electronic Publication: 2016 Nov 11.

siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.

Autor: Raspe M; Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam, the Netherlands., Kedziora KM; Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam, the Netherlands., van den Broek B; Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam, the Netherlands., Zhao Q; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands., de Jong S; Lambert Instruments B.V., Groningen, the Netherlands., Herz J; Lambert Instruments B.V., Groningen, the Netherlands., Mastop M; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, University of Amsterdam, Amsterdam, the Netherlands., Goedhart J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, University of Amsterdam, Amsterdam, the Netherlands.; Van Leeuwenhoek Centre for Advanced Microscopy, Amsterdam, the Netherlands., Gadella TW; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, University of Amsterdam, Amsterdam, the Netherlands.; Van Leeuwenhoek Centre for Advanced Microscopy, Amsterdam, the Netherlands., Young IT; Department of Imaging Physics, Delft University of Technology, Delft, the Netherlands., Jalink K; Department of Cell Biology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, University of Amsterdam, Amsterdam, the Netherlands.; Van Leeuwenhoek Centre for Advanced Microscopy, Amsterdam, the Netherlands.

Publikováno v: Nature methods [Nat Methods] 2016 Jun; Vol. 13 (6), pp. 501-4. Date of Electronic Publication: 2016 Apr 18.

A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells.

Autor: van Unen J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands., Stumpf AD; Bio-Imaging-Center/Rudolf-Virchow-Zentrum and Department of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078, Wuerzburg, Germany., Schmid B; Bio-Imaging-Center/Rudolf-Virchow-Zentrum and Department of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078, Wuerzburg, Germany., Reinhard NR; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands.; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, NL-1066 CX, Amsterdam, the Netherlands., Hordijk PL; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands.; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, NL-1066 CX, Amsterdam, the Netherlands., Hoffmann C; Bio-Imaging-Center/Rudolf-Virchow-Zentrum and Department of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078, Wuerzburg, Germany., Gadella TW Jr; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands., Goedhart J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE, Amsterdam, The Netherlands.

Publikováno v: PloS one [PLoS One] 2016 Jan 22; Vol. 11 (1), pp. e0146789. Date of Electronic Publication: 2016 Jan 22 (Print Publication: 2016).

Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization.

Autor: van Unen J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands., Reinhard NR; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands., Yin T; Center for Cell Analysis and Modeling, University of Connecticut Health Center, 400 Farmington Avenue, Farmington, CT 06032-6406., Wu YI; Center for Cell Analysis and Modeling, University of Connecticut Health Center, 400 Farmington Avenue, Farmington, CT 06032-6406., Postma M; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands., Gadella TW; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands., Goedhart J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL-1090 GE Amsterdam, The Netherlands.

Publikováno v: Scientific reports [Sci Rep] 2015 Oct 05; Vol. 5, pp. 14693. Date of Electronic Publication: 2015 Oct 05.

A local VE-cadherin and Trio-based signaling complex stabilizes endothelial junctions through Rac1.

Autor: Timmerman I; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands., Heemskerk N; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands., Kroon J; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands., Schaefer A; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands., van Rijssel J; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands., Hoogenboezem M; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands., van Unen J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Goedhart J; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Gadella TW Jr; Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Yin T; Center for Cell Analysis and Modelling, University of Connecticut Health Center, Farmington, CT 06032, USA., Wu Y; Center for Cell Analysis and Modelling, University of Connecticut Health Center, Farmington, CT 06032, USA., Huveneers S; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands., van Buul JD; Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam 1066 CX, The Netherlands j.vanbuul@sanquin.nl.

Publikováno v: Journal of cell science [J Cell Sci] 2015 Aug 15; Vol. 128 (16), pp. 3041-54. Date of Electronic Publication: 2015 Jun 26.