Structure determination of an unstable macromolecular complex enabled by nanobody-peptide bridging.

Autor: Lorentzen J; Department of Molecular Biology and Genetics, Section for Protein Science, Aarhus Universitet, Aarhus, Denmark., Pedersen DV; Department of Molecular Biology and Genetics, Section for Protein Science, Aarhus Universitet, Aarhus, Denmark., Gadeberg TAF; Department of Molecular Biology and Genetics, Section for Protein Science, Aarhus Universitet, Aarhus, Denmark., Andersen GR; Department of Molecular Biology and Genetics, Section for Protein Science, Aarhus Universitet, Aarhus, Denmark.

Publikováno v: Protein science : a publication of the Protein Society [Protein Sci] 2022 Oct; Vol. 31 (10), pp. e4432.

A Complement C3-Specific Nanobody for Modulation of the Alternative Cascade Identifies the C-Terminal Domain of C3b as Functional in C5 Convertase Activity.

Autor: Pedersen H; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Jensen RK; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Jensen JMB; Department of Clinical Immunology, DK-8200 Skejby, Denmark., Fox R; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142., Pedersen DV; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Olesen HG; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Hansen AG; Department of Biomedicine, Aarhus University, DK-8000 Aarhus, Denmark., Christiansen D; Research Laboratory, Nordland Hospital, 8092 Bodø, Norway., Mazarakis SMM; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Lojek N; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142., Hansen P; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Gadeberg TAF; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Zarantonello A; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Laursen NS; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark., Mollnes TE; Research Laboratory, Nordland Hospital, 8092 Bodø, Norway.; K.G. Jebsen Thrombosis Research and Expertise Center, University of Tromsø, 9037 Tromsø, Norway.; Department of Immunology, Oslo University Hospital, University of Oslo, 0318 Oslo, Norway.; Centre of Molecular Inflammation Research, Norwegian University of Science and Technology, 7491 Trondheim, Norway; and., Johnson MB; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142.; Howard Hughes Medical Institute, Boston Children's Hospital, Boston, MA 02115., Stevens B; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142.; Howard Hughes Medical Institute, Boston Children's Hospital, Boston, MA 02115., Thiel S; Department of Biomedicine, Aarhus University, DK-8000 Aarhus, Denmark., Andersen GR; Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark; gra@mbg.au.dk.

Publikováno v: Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2020 Oct 15; Vol. 205 (8), pp. 2287-2300. Date of Electronic Publication: 2020 Sep 16.

Structural Basis for Properdin Oligomerization and Convertase Stimulation in the Human Complement System.

Autor: Pedersen DV; Department of Molecular Biology and Genetics, Center for Structural Biology, Aarhus University, Aarhus, Denmark., Gadeberg TAF; Department of Molecular Biology and Genetics, Center for Structural Biology, Aarhus University, Aarhus, Denmark., Thomas C; Service d'Oncologie Pédiatrique, CHU Nantes, Hôpital Mère Enfant, Nantes, France., Wang Y; Department of Biology, Linderstrøm-Lang Centre for Protein Science, University of Copenhagen, Copenhagen, Denmark., Joram N; Service de Réanimation Pédiatrique, CHU Nantes, Nantes, France., Jensen RK; Department of Molecular Biology and Genetics, Center for Structural Biology, Aarhus University, Aarhus, Denmark., Mazarakis SMM; Department of Molecular Biology and Genetics, Center for Structural Biology, Aarhus University, Aarhus, Denmark., Revel M; Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, USPC, Université Paris Descartes, Université Paris Diderot, Paris, France., El Sissy C; Service d'Immunologie Biologique, Assistance Publique - Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France., Petersen SV; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Lindorff-Larsen K; Department of Biology, Linderstrøm-Lang Centre for Protein Science, University of Copenhagen, Copenhagen, Denmark., Thiel S; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Laursen NS; Department of Molecular Biology and Genetics, Center for Structural Biology, Aarhus University, Aarhus, Denmark., Fremeaux-Bacchi V; Service d'Immunologie Biologique, Assistance Publique - Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Paris, France., Andersen GR; Department of Molecular Biology and Genetics, Center for Structural Biology, Aarhus University, Aarhus, Denmark.

Publikováno v: Frontiers in immunology [Front Immunol] 2019 Aug 22; Vol. 10, pp. 2007. Date of Electronic Publication: 2019 Aug 22 (Print Publication: 2019).

A potent complement factor C3-specific nanobody inhibiting multiple functions in the alternative pathway of human and murine complement.

Autor: Jensen RK; From the Departments of Molecular Biology and Genetics and., Pihl R; Biomedicine, Aarhus University, DK-8000 Aarhus, Denmark., Gadeberg TAF; From the Departments of Molecular Biology and Genetics and., Jensen JK; From the Departments of Molecular Biology and Genetics and., Andersen KR; From the Departments of Molecular Biology and Genetics and., Thiel S; Biomedicine, Aarhus University, DK-8000 Aarhus, Denmark., Laursen NS; From the Departments of Molecular Biology and Genetics and., Andersen GR; From the Departments of Molecular Biology and Genetics and gra@mbg.au.dk.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2018 Apr 27; Vol. 293 (17), pp. 6269-6281. Date of Electronic Publication: 2018 Mar 01.