Aminoluciferins Extend Firefly Luciferase Bioluminescence into the Near-Infrared and Can Be Preferred Substrates over d-Luciferin

Autor: David M. Mofford, Gadarla Randheer Reddy, Stephen C. Miller

Publikováno v: Journal of the American Chemical Society

Firefly luciferase adenylates and oxidizes d-luciferin to chemically generate visible light and is widely used for biological assays and imaging. Here we show that both luciferase and luciferin can be reengineered to extend the scope of this light-em

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::063e52d8762ac609941d9afe878c6e3d
http://europepmc.org/articles/PMC4183640

A synthetic luciferin improves bioluminescence imaging in live mice

Autor: Gadarla Randheer Reddy, Miranda A. Paley, Stephen C. Miller, Spencer T. Adams, Melanie S. Evans, Neil Aronin, Joanna P Chaurette, Jennifer A. Prescher

Publikováno v: Evans, MS; Chaurette, JP; Adams, ST; Reddy, GR; Paley, MA; Aronin, N; et al.(2014). A synthetic luciferin improves bioluminescence imaging in live mice. Nature Methods, 11(4), 393-395. doi: 10.1038/nmeth.2839. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/4424q4b3
Nature methods, vol 11, iss 4
Nature methods

Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9fbf30407e70eccc9829e1e803854e5
http://www.escholarship.org/uc/item/4424q4b3

Synthesis and structure-activity relationships of 2-amino-1-aroylnaphthalene and 2-hydroxy-1-aroylnaphthalenes as potent antitubulin agents

Autor: Mohane Selvaraj Coumar, Chi-Yen Chang, Yi-Kun Chiang, Ching-Chuan Kuo, Uan-Kang Tan, Su-Ying Wu, Jiann-Yih Yeh, Mei-Jung Lai, Hsing Pang Hsieh, Jang Yang Chang, Jing Ping Liou, Gadarla Randheer Reddy

Publikováno v: Journal of medicinal chemistry. 51(24)

A series of aroylnaphthalene derivatives were prepared as bioisosteres of combrestatin A-4 and evaluated for anticancer activity. 2-Amino-1-aroylnaphthalene and 2-hydroxy-1-aroylnaphthalene, 9 and 8, respectively, showed strong antiproliferative acti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d234a7c92fa90567537f20661ee0ba2b
https://pubmed.ncbi.nlm.nih.gov/19053773

Identification of Mutant Firefly Luciferases that Efficiently Utilize Aminoluciferins

Autor: Katryn R. Harwood, David M. Mofford, Stephen C. Miller, Gadarla Randheer Reddy

Publikováno v: Chemistry & Biology. (12):1649-1657

Firefly luciferase-catalyzed light emission from D-luciferin is widely used as a reporter of gene expression and enzymatic activity both in vitro and in vivo. Despite the power of bioluminescence for imaging and drug discovery, light emission from fi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34cac3f3d59a07563e5244ec27faefa8