Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Gabriele Lhota"'
Autor:
Diana Széliová, Jerneja Štor, Isabella Thiel, Marcus Weinguny, Michael Hanscho, Gabriele Lhota, Nicole Borth, Jürgen Zanghellini, David E Ruckerbauer, Isabel Rocha
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 6, p e1009022 (2021)
Chinese hamster ovary (CHO) cells are the leading platform for the production of biopharmaceuticals with human-like glycosylation. The standard practice for cell line generation relies on trial and error approaches such as adaptive evolution and high
Externí odkaz:
https://doaj.org/article/3f6e3e27cbca41a8845046f16e44f498
Publikováno v:
Biotechnology progressREFERENCES. 38(5)
Glycation on lysine side chains of recombinant monoclonal antibodies (mAb) is a well-known phenomenon in manufacturing processes of biopharmaceuticals that potentially alter the efficacy of the therapeutic protein. In the present study, we report kin
Glycation on lysine side chains of recombinant monoclonal antibodies (mAb) is a well known phenomenon in manufacturing processes of biopharmaceuticals that potentially alter the efficacy of the therapeutic protein. Previously, we reported a method to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9dd1b29af97feba666a2a59263435ff8
https://doi.org/10.22541/au.163506359.97922307/v1
https://doi.org/10.22541/au.163506359.97922307/v1
Autor:
Gabriele Lhota, Diana Széliová, David E. Ruckerbauer, Nicole Borth, Jerneja Štor, Isabel Rocha, Marcus Weinguny, Isabella Thiel, Michael Hanscho, Jürgen Zanghellini
Publikováno v:
PLoS Computational Biology, Vol 17, Iss 6, p e1009022 (2021)
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
PLoS Computational Biology
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
PLoS Computational Biology
Chinese hamster ovary (CHO) cells are the leading platform for the production of biopharmaceuticals with human-like glycosylation. The standard practice for cell line generation relies on trial and error approaches such as adaptive evolution and high
Autor:
Karola Vorauer-Uhl, Gerald Striedner, Bernhard Sissolak, Gabriele Lhota, Wolfgang Sommeregger
Publikováno v:
Biotechnology Progress
Post‐translational, nonenzymatic glycation of monoclonal antibodies (mAbs) in the presence of reducing sugars (in bioprocesses) is a widely known phenomenon, which affects protein heterogeneity and potentially has an impact on quality, safety, and
Publikováno v:
Colloids and Surfaces B: Biointerfaces. 154:186-194
Non-fluidic bio-layer interferometry (BLI) has rapidly become a standard tool for monitoring almost all biomolecular interactions in a label-free, real-time and high-throughput manner. High-efficiency screening methods which measure the kinetics of l
Autor:
Karola, Vorauer-Uhl, Gabriele, Lhota
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1923
The product quality of recombinant proteins is of major importance for their intended purpose. The initial characterization of both simple and complex products should be performed as soon as practical. However, to comply with this high standard, appr
Autor:
Karola, Vorauer-Uhl, Gabriele, Lhota
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1923
Quantification of various proteins expressed in yeast can be performed by different methods. In this respect, classical as well as advanced techniques can be applied, where the analysis of crude supernatants is of special interest in screening but al